Optogenetics in Freely Moving Mammals: Dopamine and Reward.

Zhang, Feng; Tsai, Hsing-Chen; Airan, Raag D; Stuber, Garret D; Adamantidis, Antoine Roger; de Lecea, Luis; Bonci, Antonello; Deisseroth, Karl (2015). Optogenetics in Freely Moving Mammals: Dopamine and Reward. Cold Spring Harbor protocols, 2015(8), pp. 715-724. Cold Spring Harbor Laboratory 10.1101/pdb.top086330

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Brain reward systems play a central role in the cognitive and hedonic behaviors of mammals. Multiple neuron types and brain regions are involved in reward processing, posing fascinating scientific questions, and major experimental challenges. Using diverse approaches including genetics, electrophysiology, imaging, and behavioral analysis, a large body of research has focused on both normal functioning of the reward circuitry and on its potential significance in neuropsychiatric diseases. In this introduction, we illustrate a real-world application of optogenetics to mammalian behavior and physiology, delineating procedures and technologies for optogenetic control of individual components of the reward circuitry. We describe the experimental setup and protocol for integrating optogenetic modulation of dopamine neurons with fast-scan cyclic voltammetry, conditioned place preference, and operant conditioning to assess the causal role of well-defined electrical and biochemical signals in reward-related behavior.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurology

UniBE Contributor:

Adamantidis, Antoine Roger


600 Technology > 610 Medicine & health




Cold Spring Harbor Laboratory




Stefanie Hetzenecker

Date Deposited:

28 Jun 2018 14:57

Last Modified:

05 Dec 2022 15:14

Publisher DOI:


PubMed ID:




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