Serine Acetyltransferase: A Bottleneck for Cysteine and Glutathione Synthesis

Hesse, Holger; Harms, Karsten; von Ballmoos, Peter; Brunold, Christian; Willmitzer, Lothar; Höfgen, Rainer (2000). Serine Acetyltransferase: A Bottleneck for Cysteine and Glutathione Synthesis. In: Brunold, Christian; Rennenberg, Heinz; de Kok, Luit J.; Stulen, Ineke; Davidian, Jean-Claude (eds.) Sulfur Nutrition and Sulfur Assimilation in Higher Plants (pp. 321-323). Bern: Haupt Verlag

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The cysE gene encoding serine acetyltransferase from Escherichia coli, has been introduced into the genome of potato plants to investigate the role of SAT activity in plastids towards
cysteine synthesis. Transgenic lines with up to 20-fold higher SAT activity exhibited significantly higher levels of cysteine and glutathione. Expression of cysteine synthase
isoforms was unchanged while a marginal effect on activity has been observed. Physiological relevance for cysteine formation is discussed.

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