Schürmann, Peter; Brunold, Christian (1980). Formation of Cysteine from Adenosine 5′-Phosphosulfate (APS) in Extracts from Spinach Chloroplasts. Zeitschrift für Pflanzenphysiologie, 100(3), pp. 257-268. Gustav Fischer Verlag 10.1016/S0044-328X(80)80254-6
Full text not available from this repository. (Request a copy)In an assay system containing essentially extract from spinach chloroplasts, 3.3 mM glutathione, O-acetyl-L-serine, and reduced ferredoxin, 35S-cysteine was formed from 35S-adenosine 5′-phosphosulf ate (35S-APS) at a rate of 1 to 6 nmol per mg protein per hour. Addition of non-radioactive adenosine 3′-phosphate 5′-phosphosulf ate (PAPS) to the reaction mixture did not reduce appreciably the rate of 35S-cysteine formation from 35S-APS, indicating that APS is not phosphorylated to PAPS prior to reduction.
When glutathione was replaced by dithiothreitol 35S-cysteine was formed at comparable rates. The addition of different spinach thioredoxins did not influence 35S-cysteine formation. Free 35S-SO32− was detected in the reaction mixture with either glutathione or dithiothreitol as active thiol.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Stress Physiology [discontinued] 08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) |
UniBE Contributor: |
Brunold, Christian |
Subjects: |
500 Science > 580 Plants (Botany) |
ISSN: |
0044-328X |
Publisher: |
Gustav Fischer Verlag |
Language: |
English |
Submitter: |
Peter Alfred von Ballmoos-Haas |
Date Deposited: |
18 Jul 2018 11:38 |
Last Modified: |
05 Dec 2022 15:16 |
Publisher DOI: |
10.1016/S0044-328X(80)80254-6 |
Uncontrolled Keywords: |
adenosine 5′-phosphosulfate; cysteine; glutathione; spinach chloroplasts |
URI: |
https://boris.unibe.ch/id/eprint/118351 |
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