Formation of Cysteine from Adenosine 5′-Phosphosulfate (APS) in Extracts from Spinach Chloroplasts

Schürmann, Peter; Brunold, Christian (1980). Formation of Cysteine from Adenosine 5′-Phosphosulfate (APS) in Extracts from Spinach Chloroplasts. Zeitschrift für Pflanzenphysiologie, 100(3), pp. 257-268. Gustav Fischer Verlag 10.1016/S0044-328X(80)80254-6

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In an assay system containing essentially extract from spinach chloroplasts, 3.3 mM glutathione, O-acetyl-L-serine, and reduced ferredoxin, 35S-cysteine was formed from 35S-adenosine 5′-phosphosulf ate (35S-APS) at a rate of 1 to 6 nmol per mg protein per hour. Addition of non-radioactive adenosine 3′-phosphate 5′-phosphosulf ate (PAPS) to the reaction mixture did not reduce appreciably the rate of 35S-cysteine formation from 35S-APS, indicating that APS is not phosphorylated to PAPS prior to reduction. When glutathione was replaced by dithiothreitol 35S-cysteine was formed at comparable rates. The addition of different spinach thioredoxins did not influence 35S-cysteine formation. Free 35S-SO32− was detected in the reaction mixture with either glutathione or dithiothreitol as active thiol.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Stress Physiology (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Brunold, Christian

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0044-328X

Publisher:

Gustav Fischer Verlag

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

18 Jul 2018 11:38

Last Modified:

18 Jul 2018 11:38

Publisher DOI:

10.1016/S0044-328X(80)80254-6

Uncontrolled Keywords:

adenosine 5′-phosphosulfate; cysteine; glutathione; spinach chloroplasts

URI:

https://boris.unibe.ch/id/eprint/118351

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