Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response.

Wang, Junhua; Siffert, Myriam; Spiliotis, Markus; Gottstein, Bruno (2016). Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response. Journal of immunology research, 2016, p. 1450398. Hindawi Publishing Corporation 10.1155/2016/1450398

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Regulatory T (Tregs) cells play an important role in mediating tolerance to self-antigens but can also mediate detrimental tolerance to tumours and pathogens in a Foxp3-dependent manner. Genetic tools exploiting the locus including bacterial artificial chromosome- (BAC-) transgenic DEpletion of REGulatory T cells (DEREG) mice have provided essential information on Treg biology and the potential therapeutic modulation of tolerance. In DEREG mice, Foxp3 Tregs selectively express enhanced green fluorescent protein (eGFP) and diphtheria toxin (DT) receptor, allowing for the specific depletion of Tregs through DT administration. We here provide a detailed overview about an important consideration that long-term administration of DT induces a humoral immune response with an appropriate production of anti-DT antibodies that can inactivate DT and thus abrogate its effect in the DEREG mouse. Additionally, we showed that anti-DT mouse serum partially neutralized DT-induced Foxp3 inhibition.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Wang, Junhua; Siffert, Myriam; Spiliotis, Markus and Gottstein, Bruno

Subjects:

600 Technology > 630 Agriculture
600 Technology > 610 Medicine & health

ISSN:

2314-8861

Publisher:

Hindawi Publishing Corporation

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

18 Jul 2018 12:30

Last Modified:

22 Jul 2018 02:30

Publisher DOI:

10.1155/2016/1450398

PubMed ID:

28074191

BORIS DOI:

10.7892/boris.118623

URI:

https://boris.unibe.ch/id/eprint/118623

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