Production of a recombinant antigen of Echinococcus multilocularis with high immunodiagnostic sensitivity and specificity

Vogel, Monique; Gottstein, Bruno; Müller, Norbert; Seebeck, Thomas (1988). Production of a recombinant antigen of Echinococcus multilocularis with high immunodiagnostic sensitivity and specificity. Molecular and biochemical parasitology, 31(2), pp. 117-125. Elsevier 10.1016/0166-6851(88)90162-4

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A cDNA library derived from mRNA of metacestodes from Echinococcus multilocularis was constructed in the Escherichia coli expression vector lambda gt11 and screened with human patients' sera. The recombinant proteins of 11 positive phages were further evaluated for their potential as immunodiagnostic reagent. One isolated clone (from lysogen II/3) synthesized a fusion protein which was rapidly degraded. This intracellular degradation process provided two distinct polypeptides with Mr of about 31,000 and 33,000, both showing strong binding activity with specific patients' antibodies. The diagnostic value of the II/3-antigen was evaluated by mini-Western-blot with sera from 41 patients infected with E. multilocularis and sera from 77 patients with other helminthic infections, resulting in a diagnostic sensitivity of 98% and an over-all specificity of 96%. These results suggest the usefulness of the recombinant II/3-antigen for immunodiagnosis of human alveolar echinococcosis.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology, Clinical Immunology and Allergology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Vogel, Monique; Gottstein, Bruno; Müller, Norbert and Seebeck, Thomas

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture
600 Technology > 610 Medicine & health

ISSN:

0166-6851

Publisher:

Elsevier

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

18 Jul 2018 12:40

Last Modified:

18 Jul 2018 12:40

Publisher DOI:

10.1016/0166-6851(88)90162-4

PubMed ID:

3185617

URI:

https://boris.unibe.ch/id/eprint/118634

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