Echinococcus multilocularis: molecular and immunochemical characterization of diagnostic antigen II/3-10.

Felleisen, R; Gottstein, Bruno (1993). Echinococcus multilocularis: molecular and immunochemical characterization of diagnostic antigen II/3-10. Parasitology, 107(03), pp. 335-342. Cambridge University Press 10.1017/S0031182000079300

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A recombinant Echinococcus multilocularis antigen (II/3-10), which had previously been shown to exhibit immunodiagnostic characteristics highly specific for human alveolar echinococcosis, and the corresponding native parasite antigen, were further characterized with immunochemical and molecular biological methods. Immunoblot analysis using a polyclonal antiserum raised in rabbits against the recombinant protein, and subsequent Northern hybridization analysis, revealed that the native antigen was expressed by E. multilocularis at the adult as well as at the metacestode stage. In metacestodes, the antigen was shown by using indirect immunofluorescence and the same antiserum to be localized within the germinal layer and membrane structures of developing protoscolices. Electrophoretic analyses revealed remarkable differences in the apparent molecular weight of the antigen under reducing and non-reducing conditions. In further immunoblot analyses, anti-II/3-10 antibodies identified the corresponding epitopes on bands with identical M(r) in all E. multilocularis isolates investigated (European, Asian and North American). By Southern hybridization analyses of the respective gene, phylogenetically related sequences were shown to be present in other helminth species such as E. granulosus and several Taenia spp. In the same respect, immunoblotting revealed that anti-II/3-10 antibodies reacted with antigens of different M(r) from various E. granulosus isolates and some other cestode species, indicating the presence of shared and thus potentially cross-reacting epitopes. The relevance of these findings for the immunodiagnostic performance of the recombinant antigen is discussed.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Gottstein, Bruno

Subjects:

600 Technology > 630 Agriculture
600 Technology > 610 Medicine & health

ISSN:

0031-1820

Publisher:

Cambridge University Press

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

18 Jul 2018 13:52

Last Modified:

18 Jul 2018 16:26

Publisher DOI:

10.1017/S0031182000079300

PubMed ID:

7694224

BORIS DOI:

10.7892/boris.118674

URI:

https://boris.unibe.ch/id/eprint/118674

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