Electron microscopical investigation of surface alterations on Giardia lamblia trophozoites after exposure to a cytotoxic monoclonal antibody.

Hemphill, Andrew; Stäger, S; Gottstein, Bruno; Müller, Norbert (1996). Electron microscopical investigation of surface alterations on Giardia lamblia trophozoites after exposure to a cytotoxic monoclonal antibody. Parasitology research, 82(3), pp. 206-210. Springer-Verlag 10.1007/s004360050096

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The present study describes a transmission electron microscopical investigation of trophozoites from Giardia lamblia clone GS/M-83-H7 after exposure to monoclonal antibody (MAb) G10/4. From previous studies it is known that this antibody immunoreacts with the parasite's major surface antigen VSP (variable surface protein) and exhibits a complement-independent cytotoxic effect on trophozoites of clone GS/M-83-H7. Our investigations revealed that cytotoxicity of MAb G10/4 is associated with shedding of VSP-containing membrane vesicles from the parasite surface and a concomitant partial disruption of the cellular membrane. These morphological alterations depend on the cross-linking capacity of the antibody because the immunoreactivity of respective monovalent F(ab)' has no significant influence on the cell-surface structure. These findings indicate that the membrane-disintegrative activity of MAb G10/4 may be responsible for the parasitocidal function of the antibody.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Hemphill, Andrew; Gottstein, Bruno and Müller, Norbert

Subjects:

600 Technology > 630 Agriculture
600 Technology > 610 Medicine & health

ISSN:

0932-0113

Publisher:

Springer-Verlag

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

18 Jul 2018 14:13

Last Modified:

18 Jul 2018 16:25

Publisher DOI:

10.1007/s004360050096

PubMed ID:

8801550

BORIS DOI:

10.7892/boris.118698

URI:

https://boris.unibe.ch/id/eprint/118698

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