Adhesion and invasion of bovine endothelial cells by Neospora caninum.

Hemphill, A; Gottstein, Bruno; Kaufmann, H (1996). Adhesion and invasion of bovine endothelial cells by Neospora caninum. Parasitology, 112(02), pp. 183-197. Cambridge University Press 10.1017/S0031182000084754

Preview

Text S0031182000084754.pdf - Published Version Available under License Publisher holds Copyright. Download (10MB) | Preview

Neospora caninum is a recently identified coccidian parasite which was, until 1988, misdiagnosed as Toxoplasma gondii. It causes paralysis and death in dogs and neonatal mortality and abortion in cattle, sheep, goats and horses. The life-cycle of Neospora has not yet been elucidated. The only two stages identified so far are tissue cysts and intracellularly dividing tachyzoites. Very little is known about the biology of this species. We have set up a fluorescence-based adhesion/invasion assay in order to investigate the interaction of N. caninum tachyzoites with bovine aorta endothelial (BAE) cells in vitro. Treatment of both host cells and parasites with metabolic inhibitors determined the metabolic requirements for adhesion and invasion. Chemical and enzymatic modifications of parasite and endothelial cell surfaces were used in order to obtain information on the nature of cell surface components responsible for the interaction between parasite and host. Electron microscopical investigations defined the ultrastructural characteristics of the adhesion and invasion process, and provided information on the intracellular development of the parasites.

Interest & Impact

Downloads

295 since deposited on 19 Jul 2018
43 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item