Adhesion and invasion of bovine endothelial cells by Neospora caninum.

Hemphill, A; Gottstein, Bruno; Kaufmann, H (1996). Adhesion and invasion of bovine endothelial cells by Neospora caninum. Parasitology, 112(02), pp. 183-197. Cambridge University Press 10.1017/S0031182000084754

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Neospora caninum is a recently identified coccidian parasite which was, until 1988, misdiagnosed as Toxoplasma gondii. It causes paralysis and death in dogs and neonatal mortality and abortion in cattle, sheep, goats and horses. The life-cycle of Neospora has not yet been elucidated. The only two stages identified so far are tissue cysts and intracellularly dividing tachyzoites. Very little is known about the biology of this species. We have set up a fluorescence-based adhesion/invasion assay in order to investigate the interaction of N. caninum tachyzoites with bovine aorta endothelial (BAE) cells in vitro. Treatment of both host cells and parasites with metabolic inhibitors determined the metabolic requirements for adhesion and invasion. Chemical and enzymatic modifications of parasite and endothelial cell surfaces were used in order to obtain information on the nature of cell surface components responsible for the interaction between parasite and host. Electron microscopical investigations defined the ultrastructural characteristics of the adhesion and invasion process, and provided information on the intracellular development of the parasites.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Gottstein, Bruno

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0031-1820

Publisher:

Cambridge University Press

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

19 Jul 2018 15:31

Last Modified:

19 Jul 2018 15:31

Publisher DOI:

10.1017/S0031182000084754

PubMed ID:

8851858

BORIS DOI:

10.7892/boris.118706

URI:

https://boris.unibe.ch/id/eprint/118706

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