Diagnosis of Neospora caninum and Toxoplasma gondii infection by PCR and DNA hybridization immunoassay.

Müller, Norbert; Zimmermann, V; Hentrich, B; Gottstein, Bruno (1996). Diagnosis of Neospora caninum and Toxoplasma gondii infection by PCR and DNA hybridization immunoassay. Journal of clinical microbiology, 34(11), pp. 2850-2852. American Society for Microbiology

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A recently described PCR test for the identification of Neospora caninum and Toxoplasma gondii has been further developed and optimized in view of its practicability for routine diagnostic application. The N. caninum-specific PCR was adapted to the diagnostic operating standard of the T. gondii-specific PCR in that the uracil DNA glycosidase system was introduced, which eliminates potential carry-over contaminations of amplified target DNA from previous reactions. Furthermore, both PCR tests were optimized by including a DNA hybridization immunoassay based on the use of the commercially available Gen-eti-k DEIA kit. This assay allowed highly sensitive and specific detection of respective DNA amplification products and thus substantially facilitated the reading and interpretation of the test results.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
UniBE Contributor:	Müller, Norbert, Gottstein, Bruno
Subjects:	600 Technology > 610 Medicine & health 600 Technology > 630 Agriculture
ISSN:	0095-1137
Publisher:	American Society for Microbiology
Language:	English
Submitter:	Bruno Gottstein
Date Deposited:	23 Jul 2018 09:25
Last Modified:	05 Dec 2022 15:16
PubMed ID:	8897199
URI:	https://boris.unibe.ch/id/eprint/118712