Systemic and local antibody response in mice induced by a recombinant peptide fragment from Giardia lamblia variant surface protein (VSP) H7 produced by a Salmonella typhimurium vaccine strain.

Stäger, S; Gottstein, Bruno; Müller, Norbert (1997). Systemic and local antibody response in mice induced by a recombinant peptide fragment from Giardia lamblia variant surface protein (VSP) H7 produced by a Salmonella typhimurium vaccine strain. International journal for parasitology, 27(8), pp. 965-971. Elsevier

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Previous experimental infections of mice with the intestinal protozoan Giardia lamblia had revealed that antigenic variation of the parasite was associated with the major surface antigen, named variant surface protein (VSP). In the present study, a gene segment of the VSP (VSPH7) from the well-characterized G. lamblia clone GS/M-83-H7 was expressed in the live-attenuated Salmonella typhimurium vaccine strain LT2M1C. The recombinant vaccine was assessed for its potential to induce both a systemic and a local antibody response in mice. Peroral administration of the vaccine stimulated synthesis of serum IgG and intestinal IgA antibodies directed against Salmonella antigens as well as against VSPH7. With respect to the anti-VSPH7 antibody concentrations, vaccination of animals resulted in systemic and local antibody responses similar to those induced by experimental or natural infections of mice with G. lamblia clone GS/M-83-H7. Subclass specification of serum anti-VSPH7 IgG demonstrated THelper 2-cell dependent IgG1- and/or IgG2b-type antibody production. No significant THelper 1-cell dependent IgG2a-type anti-VSPH7 antibody production was detected in infected or in vaccinated animals. Taken together, these data indicate a strong intrinsic antigenicity of VSPH7, which stimulates a THelper 2-cell pathway of the murine immune system, independent of the route of antigen administration. Furthermore, the high immunostimulatory potential of the recombinant Salmonella/VSPH7 model vaccine suggests application of LT2M1C as an enteric biocarrier for the identification of putative new target vaccines in giardiasis.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
UniBE Contributor:	Gottstein, Bruno, Müller, Norbert
Subjects:	600 Technology > 610 Medicine & health 600 Technology > 630 Agriculture
ISSN:	0020-7519
Publisher:	Elsevier
Language:	English
Submitter:	Bruno Gottstein
Date Deposited:	23 Jul 2018 09:14
Last Modified:	05 Dec 2022 15:16
PubMed ID:	9292314
URI:	https://boris.unibe.ch/id/eprint/118721