Sequence and immunogenicity of the Taenia saginata homologue of the major surface antigen of Echinococcus spp.

Benitez, L; Harrison, L J; Parkhouse, R M; Gonzalez, L M; Gottstein, Bruno; Garate, T (1998). Sequence and immunogenicity of the Taenia saginata homologue of the major surface antigen of Echinococcus spp. Parasitology research, 84(5), pp. 426-431. Springer-Verlag

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A clone (R-Tso18) was isolated from a Taenia saginata oncosphere cDNA library by screening with sera from rabbits immunised with oncosphere extract. It contained a full-length cDNA sequence of 1893 bp with an open reading frame of 1680 bp, corresponding to 559 amino acids with a deduced molecular mass of 65.173 kDa and an isoelectric point of 6.08. The R-Tso18 protein showed 80-84% nucleotide identity with the major protoscolex surface antigens of Echinococcus multilocularis (EM10) and E. granulosus (EG10). Preliminary immunogenicity studies employing the radiolabeled R-Tso18 protein in immune co-precipitation assays indicated sero-positivity for T. saginata-infected calf sera (6/13), T. solium cysticercosis human (7/22) and pig (2/2) sera and E. multilocularis (6/10)- and E. granulosus (1/12)-infected human sera, whereas other helminth-infection sera were negative. As immuno-precipitation is a relatively insensitive assay, it was concluded that further studies on the diagnostic potential of the purified recombinant R-Tso18 antigen, or its peptides, are merited.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Gottstein, Bruno

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0932-0113

Publisher:

Springer-Verlag

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

19 Jul 2018 15:45

Last Modified:

19 Jul 2018 15:45

PubMed ID:

9610644

URI:

https://boris.unibe.ch/id/eprint/118736

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