Identification of a differentially expressed Echinococcus multilocularis protein Em6 potentially related to antigen 5 of Echinococcus granulosus.

Siles-Lucas, M; Gottstein, Bruno; Felleisen, R S (1998). Identification of a differentially expressed Echinococcus multilocularis protein Em6 potentially related to antigen 5 of Echinococcus granulosus. Parasite immunology, 20(10), pp. 473-481. Blackwell Scientific Publications

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By a strategy of differential immunological screening of an expression library constructed from adult Echinococcus multilocularis parasites, a partial cDNA sequence encoding a protein termed Em6 was isolated. This molecule displayed high sequence homology to the recombinant antigen 'Eg6' which was previously described as an immunogenic epitope of antigen 5 of E. granulosus. Further Em6 sequences and the corresponding sequences from a cattle isolate of E. granulosus were obtained by a PCR approach. By immunoblot analyses using affinity purified antibodies, expression of Em6 in fertile cysts producing protoscoleces of the E. multilocularis metacestode stage was observed. However, Em6 was absent in non-fertile metacestodes. The demonstration of a protein in E. multilocularis displaying identities to 'antigen 6' of E. granulosus could potentially contribute to the future elucidation of the relationship between antigen 5 and 'antigen 6' in the genus Echinococcus, and shed some lights on the performance of serodiagnostic assays for hydatid disease based on the respective antigens.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Gottstein, Bruno

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0141-9838

Publisher:

Blackwell Scientific Publications

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

19 Jul 2018 15:26

Last Modified:

19 Jul 2018 15:26

PubMed ID:

9797508

URI:

https://boris.unibe.ch/id/eprint/118739

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