Differential immunodiagnosis between cystic hydatid disease and other cross-reactive pathologies.

Poretti, D; Felleisen, E; Grimm, F; Pfister, M; Teuscher, F; Zuercher, C; Reichen, J; Gottstein, Bruno (1999). Differential immunodiagnosis between cystic hydatid disease and other cross-reactive pathologies. The American Journal of Tropical Medicine and Hygiene, 60(2), pp. 193-198. American Society of Tropical Medicine and Hygiene

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We assessed an Echinococcus granulosus hydatid fluid antigen-ELISA (EgHF-ELISA) as a serologic prescreening test for E. granulosus infections, supplemented by more specific confirmatory tests, including arc-5 immunoprecipitation and antigen B subunit 8-kD immunoblotting. The diagnostic sensitivity of the EgHF-ELISA was 91%. With regard to the test specificity of the EgHF-ELISA (overall = 82%), we observed relatively frequent cross-reactions in tumor patients (6%) and in patients with other parasitic diseases. Cestode-related cross-reactivity can be resolved by the complementary use of E. multilocularis-specific antigens or Taenia solium cysticercosis-specific immunoblotting. Immunoblotting based upon the detection of antibody reactivity to the 8-kD antigen of EgHF, or if appropriately detectable, to the 29-kD and 34-kD bands exhibited a 91% diagnostic sensitivity and an overall specificity of 97% or 94%, respectively. Thus, immunoblotting provided a 99% discrimination between seropositive pre-operative cystic hydatid disease cases and cross-reactive non-cestode parasitic infections or malignancies.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Gottstein, Bruno

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0002-9637

Publisher:

American Society of Tropical Medicine and Hygiene

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

19 Jul 2018 16:20

Last Modified:

19 Jul 2018 16:20

PubMed ID:

10072135

URI:

https://boris.unibe.ch/id/eprint/118771

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