An intact laminated layer is important for the establishment of secondary Echinococcus multilocularis infection.

Gottstein, Bruno; Dai, Wen Juan; Walker, Mirjam; Stettler, Marianne; Müller, Norbert; Hemphill, Andrew (2002). An intact laminated layer is important for the establishment of secondary Echinococcus multilocularis infection. Parasitology research, 88(9), pp. 822-828. Springer-Verlag 10.1007/s00436-002-0659-7

[img]
Preview
Text
Gottstein2002_Article_AnIntactLaminatedLayerIsImport.pdf - Published Version
Available under License Publisher holds Copyright.

Download (173kB) | Preview

Echinococcus multilocularis causes alveolar echinococcosis primarily in rodents, but also in humans where it represents one of the most lethal helmintic infections. We used a susceptible mouse (C57BL/6) model to demonstrate failure in controlling secondary infection with the E. multilocularis metacestode, even when performed at the lowest possible infection dose. This was achieved by intraperitoneal or intrahepatic inoculation of a single parasite vesicle. In secondary infections, the primary physical barrier between the parasite and the host is constituted by the acellular laminated layer (LL), which is predominantly composed of high-molecular-weight glycans and surrounds the entire metacestode. Only those metacestode structures which exhibited an intact LL were successful in establishing infection, whereas metacestodes which were punctured - thus exhibiting an opened LL and thereby an accessible germinal layer - were no longer infective. Conversely, both types of vesicle survived in vivo maintenance, as assessed by RT-PCR based upon II/3 gene expression. In consequence, the encapsulating LL appears to be one of the key factors that mediates survival and successful proliferation of the parasite metacestode in vivo.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Gottstein, Bruno; Müller, Norbert and Hemphill, Andrew

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0932-0113

Publisher:

Springer-Verlag

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

19 Jul 2018 15:50

Last Modified:

19 Jul 2018 15:59

Publisher DOI:

10.1007/s00436-002-0659-7

PubMed ID:

12172814

BORIS DOI:

10.7892/boris.118824

URI:

https://boris.unibe.ch/id/eprint/118824

Actions (login required)

Edit item Edit item
Provide Feedback