Regulation of Thiol Contents in Maize Roots by Intermediates and Effectors of Glutathione Synthesis

Farago, Slobodan; Brunold, Christian (1994). Regulation of Thiol Contents in Maize Roots by Intermediates and Effectors of Glutathione Synthesis. Journal of Plant Physiology, 144(4-5), pp. 433-437. Elsevier 10.1016/S0176-1617(11)82119-X

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The regulation of the synthesis and the levels of GSH and other acid-soluble thiols was studied in maize roots by applying GSH, GSH monoethylester, OTC (L-2-oxothiazolidine-4-carboxylic acid), the GSH precursors cysteine and γ-EC (γ-glutamylcysteine), the γ-EC synthetase (EC 6.3.2.2) inhibitor BSO (buthionine S,R-sulfoximine), and the herbicide safener benoxacor (4-dichloroacetyl-3,4-dihydro-3methyl- 2H-1,4-benzooxazine).

OTC, GSH and GSH monoethylester did not affect the GSH content, whereas exogenous cysteine and γ-EC augmented it 1.6- and 2.5-fold, respectively. Both cysteine and γ-EC strongly increased their endogenous level. Benoxacor increased the activity of adenosine 5' -phosphosulfate sulfotransferase and γ-EC synthetase within 2 and 4 h, respectively, but did not affect GSH synthetase activity (EC 6.3.2.3). It rapidly induced a high level of γ-EC and doubled the cysteine and the GSH contents. When benoxacor and γ-EC were applied together an additional increase in GSH was detected. BSO alone or together with benoxacor resulted in decreased GSH levels. Both substances combined caused an accumulation of γ-EC. These results are discussed on the basis of the localization of the GSH synthesizing enzymes in the proplastids and the cytoplasm and the intracellular site of action of benoxacor and BSO.

Item Type:	Journal Article (Original Article)
Division/Institute:	08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Stress Physiology [discontinued] 08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
UniBE Contributor:	Brunold, Christian
Subjects:	500 Science > 580 Plants (Botany)
ISSN:	0176-1617
Publisher:	Elsevier
Language:	English
Submitter:	Peter Alfred von Ballmoos-Haas
Date Deposited:	26 Jul 2018 09:45
Last Modified:	05 Dec 2022 15:16
Publisher DOI:	10.1016/S0176-1617(11)82119-X
URI:	https://boris.unibe.ch/id/eprint/118961