Haller, Elisabeth; Suter, Marianne; Brunold, Christian (1986). Regulation of ATP-sulfurylase and Adenosine 5'-phosphosulfate Sulfotransferase by the Sulfur and the Nitrogen Source in Heterotrophic Cell Suspension Cultures of Paul's Scarlet Rose. Journal of Plant Physiology, 125(3-4), pp. 275-283. Elsevier 10.1016/S0176-1617(86)80150-X
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The effect of the sulfur and the nitrogen source on the activity of adenosine 5'-phosphosulfate sulfotransferase (APSSTase) from cell suspension cultures of Rosa sp.(Paul's Scarlet rose) was studied.ATP-sulfurylase (EC 2.7.7.4), nitrate reductase (EC 1.6.6.1) and O-acetyl-L-serine sulfhydrylase (EC 4.2.99.9) were measured for comparison.APSSTase activity was increased by 200% after omission of a sulfur source and by 50% when cells were cultivated with NH4+ instead of NO3-.Lack of a nitrogen source or cultivation with H2S or cysteine as sulfur sources induced a decrease in APSST ase activity to a very low level.The decrease induced by H2S and cysteine was less pronounced in the presence of NH4+.ATP-sulfurylase was increased by 100% in cultures without a sulfur source, whereas lack of a nitrogen source or NH4+ instead of NO3- had no effect on its activity during 24 h.Cysteine decreased this enzyme activity by 40% after 24h.Taken together our results are consistent with the idea that APSSTase plays a central role in regulating sulfate assimilation and in coordinating it with nitrate assimilation, whereas A TP-sulfurylase seems to have an accessory function in these respects.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Stress Physiology [discontinued] 08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) |
UniBE Contributor: |
Suter, Marianne, Brunold, Christian |
Subjects: |
500 Science > 580 Plants (Botany) |
ISSN: |
0176-1617 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Peter Alfred von Ballmoos-Haas |
Date Deposited: |
27 Jul 2018 13:42 |
Last Modified: |
05 Dec 2022 15:16 |
Publisher DOI: |
10.1016/S0176-1617(86)80150-X |
Uncontrolled Keywords: |
Rosa sp.; adenosine 5'-phosphosulfate sulfotransferase; ATP-sulfurylase; nitrate reductase; regulation of sulfate assimilation |
BORIS DOI: |
10.7892/boris.118968 |
URI: |
https://boris.unibe.ch/id/eprint/118968 |
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