Regulation of ATP-sulfurylase and Adenosine 5'-phosphosulfate Sulfotransferase by the Sulfur and the Nitrogen Source in Heterotrophic Cell Suspension Cultures of Paul's Scarlet Rose

Haller, Elisabeth; Suter, Marianne; Brunold, Christian (1986). Regulation of ATP-sulfurylase and Adenosine 5'-phosphosulfate Sulfotransferase by the Sulfur and the Nitrogen Source in Heterotrophic Cell Suspension Cultures of Paul's Scarlet Rose. Journal of Plant Physiology, 125(3-4), pp. 275-283. Elsevier 10.1016/S0176-1617(86)80150-X

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The effect of the sulfur and the nitrogen source on the activity of adenosine 5'-phosphosulfate sulfotransferase (APSSTase) from cell suspension cultures of Rosa sp.(Paul's Scarlet rose) was studied.ATP-sulfurylase (EC 2.7.7.4), nitrate reductase (EC 1.6.6.1) and O-acetyl-L-serine sulfhydrylase (EC 4.2.99.9) were measured for comparison.APSSTase activity was increased by 200% after omission of a sulfur source and by 50% when cells were cultivated with NH4+ instead of NO3-.Lack of a nitrogen source or cultivation with H2S or cysteine as sulfur sources induced a decrease in APSST ase activity to a very low level.The decrease induced by H2S and cysteine was less pronounced in the presence of NH4+.ATP-sulfurylase was increased by 100% in cultures without a sulfur source, whereas lack of a nitrogen source or NH4+ instead of NO3- had no effect on its activity during 24 h.Cysteine decreased this enzyme activity by 40% after 24h.Taken together our results are consistent with the idea that APSSTase plays a central role in regulating sulfate assimilation and in coordinating it with nitrate assimilation, whereas A TP-sulfurylase seems to have an accessory function in these respects.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Stress Physiology (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Suter, Marianne and Brunold, Christian

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0176-1617

Publisher:

Elsevier

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

27 Jul 2018 13:42

Last Modified:

24 Oct 2019 20:00

Publisher DOI:

10.1016/S0176-1617(86)80150-X

Uncontrolled Keywords:

Rosa sp.; adenosine 5'-phosphosulfate sulfotransferase; ATP-sulfurylase; nitrate reductase; regulation of sulfate assimilation

BORIS DOI:

10.7892/boris.118968

URI:

https://boris.unibe.ch/id/eprint/118968

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