Method for the measurement of adenosine 5'-phosphosulfate sulfotransferase and its application for the detection of de novosynthesis of the enzyme

Brunold, Christian; Suter, Marianne (1983). Method for the measurement of adenosine 5'-phosphosulfate sulfotransferase and its application for the detection of de novosynthesis of the enzyme. Botanica Helvetica, 93(1), pp. 105-114. SP Birkhäuser Verlag Basel 10.5169/seals-65240

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A method for the measurement of adenosine 5'-phosphosulfate sulfotransferase is described with which a determination can be performed in about 2 min. This method was used for examining the increase in adenosine 5' -phosphosulfate sulfotransferase activity of Lemna minor which was observed when these organisms were cultivated with NH~ and NO3 instead of NO3 as a nitrogen source. 15N-density Iabelling and subsequent density gradient centrifugation showed that this increase is at least initially due to an increased de novo-synthesis.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Stress Physiology (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Brunold, Christian and Suter, Marianne

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0253-1453

Publisher:

SP Birkhäuser Verlag Basel

Language:

German

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

27 Jul 2018 14:24

Last Modified:

21 Nov 2019 00:35

Publisher DOI:

10.5169/seals-65240

BORIS DOI:

10.7892/boris.118975

URI:

https://boris.unibe.ch/id/eprint/118975

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