Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

Ficht, Xenia Maria; Stolp, Bettina; Thelen, Flavian; Stein, Jens Volker (2018). Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy. Journal of visualized experiments(135) MYJoVE Corporation 10.3791/57283

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The submandibular salivary gland (SMG) is one of the three major salivary glands, and is of interest for many different fields of biological research, including cell biology, oncology, dentistry, and immunology. The SMG is an exocrine gland comprised of secretory epithelial cells, myofibroblasts, endothelial cells, nerves, and extracellular matrix. Dynamic cellular processes in the rat and mouse SMG have previously been imaged, mostly using inverted multi-photon microscope systems. Here, we describe a straightforward protocol for the surgical preparation and stabilization of the murine SMG in anesthetized mice for in vivo imaging with upright multi-photon microscope systems. We present representative intravital image sets of endogenous and adoptively transferred fluorescent cells, including the labeling of blood vessels or salivary ducts and second harmonic generation to visualize fibrillar collagen. In sum, our protocol allows for surgical preparation of mouse salivary glands in upright microscopy systems, which are commonly used for intravital imaging in the field of immunology.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute
09 Interdisciplinary Units > Microscopy Imaging Center MIC

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Ficht, Xenia Maria; Stolp, Bettina; Thelen, Flavian and Stein, Jens Volker

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

1940-087X

Publisher:

MYJoVE Corporation

Language:

English

Submitter:

Xenia Maria Ficht

Date Deposited:

17 Jan 2019 18:02

Last Modified:

18 Jan 2019 07:40

Publisher DOI:

10.3791/57283

PubMed ID:

29781999

URI:

https://boris.unibe.ch/id/eprint/118990

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