Molecular analysis of isolates from symptomatic and asymptomatic children from La Habana, Cuba.

Jerez Puebla, Luis Enrique; Núñez, Fidel A; Santos, Lissette Pérez; Rivero, Lázara Rojas; Silva, Isabel Martínez; Valdés, Lucía Ayllón; Millán, Iraís Atencio; Müller, Norbert (2017). Molecular analysis of isolates from symptomatic and asymptomatic children from La Habana, Cuba. Parasite epidemiology and control, 2(3), pp. 105-113. Elsevier 10.1016/j.parepi.2017.05.003

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Giardiasis is considered the most common intestinal parasitic disease in humans worldwide. In Cuba, this infection has particularly a strong clinical impact on the child population. is a highly diverse protozoan, which comprises a complex of eight morphologically identical genetic assemblages, further divided into sub-assemblages. The present study used triose phosphate isomerase () and small-subunit ribosomal RNA (SSU rRNA) genes as genetic markers for the identification of assemblages and sub-assemblages in correlation with clinical and epidemiological data in children attended at the Paediatric Hospital "William Soler" and at Pedro Kouri Institute, between 2015 and 2016. A prevalence of 8% of infection was recorded in stool samples after concentration techniques from 68 children out of 847 analysed. A 100% detection of DNA was achieved by a SSU-rRNA PCR, whereas DNA from 63 of 68 (92.6%) was successfully amplified by -PCR. By this assemblage-specific -PCR 32 (50.8%) assemblage B, 17 (27.0%) assemblage A and 14 (22.2%) mixed infection (A + B) were identified. Assemblage B was significantly ( < 0.02) more frequently found in children with diarrhoea. Sequence analysis of the gene of isolates from symptomatic children showed that assemblage A belonged to the sub-assemblage AII, and 4 sub assemblages BIV and 1 sub assemblage BIII were also recorded. Only 2 discordant genotyping results were observed by phylogenetic comparison of SSU-rRNA and sequences. Further studies with novel molecular tools for a better discrimination at the sub-assemblage level are needed to identify the dynamics of spread of giardiasis and to verify possible correlations between genetic diversity and clinical manifestation.

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