Molecular analysis of isolates from symptomatic and asymptomatic children from La Habana, Cuba.

Jerez Puebla, Luis Enrique; Núñez, Fidel A; Santos, Lissette Pérez; Rivero, Lázara Rojas; Silva, Isabel Martínez; Valdés, Lucía Ayllón; Millán, Iraís Atencio; Müller, Norbert (2017). Molecular analysis of isolates from symptomatic and asymptomatic children from La Habana, Cuba. Parasite epidemiology and control, 2(3), pp. 105-113. Elsevier 10.1016/j.parepi.2017.05.003

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Giardiasis is considered the most common intestinal parasitic disease in humans worldwide. In Cuba, this infection has particularly a strong clinical impact on the child population. is a highly diverse protozoan, which comprises a complex of eight morphologically identical genetic assemblages, further divided into sub-assemblages. The present study used triose phosphate isomerase () and small-subunit ribosomal RNA (SSU rRNA) genes as genetic markers for the identification of assemblages and sub-assemblages in correlation with clinical and epidemiological data in children attended at the Paediatric Hospital "William Soler" and at Pedro Kouri Institute, between 2015 and 2016. A prevalence of 8% of infection was recorded in stool samples after concentration techniques from 68 children out of 847 analysed. A 100% detection of DNA was achieved by a SSU-rRNA PCR, whereas DNA from 63 of 68 (92.6%) was successfully amplified by -PCR. By this assemblage-specific -PCR 32 (50.8%) assemblage B, 17 (27.0%) assemblage A and 14 (22.2%) mixed infection (A + B) were identified. Assemblage B was significantly ( < 0.02) more frequently found in children with diarrhoea. Sequence analysis of the gene of isolates from symptomatic children showed that assemblage A belonged to the sub-assemblage AII, and 4 sub assemblages BIV and 1 sub assemblage BIII were also recorded. Only 2 discordant genotyping results were observed by phylogenetic comparison of SSU-rRNA and sequences. Further studies with novel molecular tools for a better discrimination at the sub-assemblage level are needed to identify the dynamics of spread of giardiasis and to verify possible correlations between genetic diversity and clinical manifestation.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Müller, Norbert

Subjects:

600 Technology > 630 Agriculture

ISSN:

2405-6731

Publisher:

Elsevier

Language:

English

Submitter:

Monika Mumenthaler

Date Deposited:

21 Aug 2018 12:17

Last Modified:

25 Oct 2019 07:18

Publisher DOI:

10.1016/j.parepi.2017.05.003

PubMed ID:

29774288

Uncontrolled Keywords:

Assemblages Children Cuba Giardia duodenalis SSU-rRNA Sequences tpi

BORIS DOI:

10.7892/boris.119373

URI:

https://boris.unibe.ch/id/eprint/119373

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