Jonsdottir, Hulda Run; Dijkman, Ronald (2015). Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures. In: Jane Maier, Helena; Bickerton, Erica; Britton, Paul (eds.) Coronaviruses. Methods in Molecular Biology: Vol. 1282 (pp. 73-87). Springer 10.1007/978-1-4939-2438-7_8
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The human airway serves as the entry point of human respiratory viruses, including human coronaviruses. In this chapter we outline the methods by which we establish fully differentiated airway epithelium and its use for human coronavirus propagation. Additionally, we outline methods for immunofluorescence staining of these cultures for virus detection, characterization of cell tropism, and how to perform antiviral assays and quantify viral replication.
Item Type: |
Book Section (Book Chapter) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) |
UniBE Contributor: |
Jonsdottir, Hulda Run, Dijkman, Ronald |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
1940-6029 |
ISBN: |
978-1-4939-2437-0 |
Series: |
Methods in Molecular Biology |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Barbara Bach |
Date Deposited: |
22 Aug 2018 14:04 |
Last Modified: |
05 Dec 2022 15:17 |
Publisher DOI: |
10.1007/978-1-4939-2438-7_8 |
PubMed ID: |
25720473 |
BORIS DOI: |
10.7892/boris.119383 |
URI: |
https://boris.unibe.ch/id/eprint/119383 |
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