Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures.

Jonsdottir, Hulda Run; Dijkman, Ronald (2015). Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures. In: Jane Maier, Helena; Bickerton, Erica; Britton, Paul (eds.) Coronaviruses. Methods in Molecular Biology: Vol. 1282 (pp. 73-87). Springer 10.1007/978-1-4939-2438-7_8

[img] Text
Seiten aus 2015_Book_Coronaviruses.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (233kB) | Request a copy

The human airway serves as the entry point of human respiratory viruses, including human coronaviruses. In this chapter we outline the methods by which we establish fully differentiated airway epithelium and its use for human coronavirus propagation. Additionally, we outline methods for immunofluorescence staining of these cultures for virus detection, characterization of cell tropism, and how to perform antiviral assays and quantify viral replication.

Item Type:

Book Section (Book Chapter)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Jonsdottir, Hulda Run and Dijkman, Ronald

Subjects:

600 Technology > 630 Agriculture

ISSN:

1940-6029

ISBN:

978-1-4939-2437-0

Series:

Methods in Molecular Biology

Publisher:

Springer

Language:

English

Submitter:

Monika Mumenthaler

Date Deposited:

22 Aug 2018 14:04

Last Modified:

22 Aug 2018 14:04

Publisher DOI:

10.1007/978-1-4939-2438-7_8

PubMed ID:

25720473

BORIS DOI:

10.7892/boris.119383

URI:

https://boris.unibe.ch/id/eprint/119383

Actions (login required)

Edit item Edit item
Provide Feedback