A potential test system for detecting contaminations by bacterial lipoproteins.

Farhat, Katja; Ulmer, Artur J; Jungi, Thomas (2012). A potential test system for detecting contaminations by bacterial lipoproteins. Veterinary immunology and immunopathology, 145(1-2), pp. 66-73. Elsevier 10.1016/j.vetimm.2011.10.009

[img] Text
1-s2.0-S0165242711004168-main.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (591kB) | Request a copy

Biological specimens are often contaminated with bacteria-derived products such as LPS or lipoproteins (LP), which trigger unwanted inflammatory responses in hosts. Whereas a contamination by LPS can be determined by various test systems, a contamination by LP can as yet not be determined. TLR4 and TLR2 are key components of the LPS and the LP receptor complex, respectively. It was tested in this study whether HEK293 cell stably transfected with bovine TLR2 have the ability to react to low concentrations of diacylated and triacylated synthetic LP. The stable cell lines we present here recognize low concentrations of synthetic LP resembling LP of different bacteria. Therefore, these cells are suitable to detect low contaminations present in probes. For example, HEK293 cells stably transfected with bovine TLR2 recognized an egg albumin preparation as contaminated, as evidenced by copious production of IL-8. In contrast, these cells did not respond to a highly purified human serum albumin (HSA) preparation used in the clinic but responded to HSA containing small amounts of diacylated synthetic LP. The TLR4 ligand LPS is often said to activate TLR2. Here we present evidence that LP contaminations are responsible for TLR2 activity. HEK293 cells stably transfected with bovine TLR2 and TLR1 (e.g. clone 1) did not respond to ultra-pure Escherichia coli LPS preparations but acquired responsiveness when stimulated with differently purified commercial LPS. Thus, the described system involving HEK293 cells stably transfected with bovine TLR2 and TLR1 is the first test system described attempting to measure a contamination by LP.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Jungi, Thomas

Subjects:

600 Technology > 630 Agriculture

ISSN:

0165-2427

Publisher:

Elsevier

Language:

English

Submitter:

Monika Mumenthaler

Date Deposited:

21 Aug 2018 12:57

Last Modified:

21 Aug 2018 12:57

Publisher DOI:

10.1016/j.vetimm.2011.10.009

PubMed ID:

22133281

BORIS DOI:

10.7892/boris.119390

URI:

https://boris.unibe.ch/id/eprint/119390

Actions (login required)

Edit item Edit item
Provide Feedback