Boosting effect of IL-7 in interferon gamma release assays to diagnose Mycobacterium tuberculosis infection.

Hiza, Hellen; Fenner, Lukas; Hella, Jerry; Kuchaka, Davis; Sasamalo, Mohamed; Blauenfeldt, Thomas; Kibiki, Gibson; Kavishe, Reginald A; Mhimbira, Francis; Ruhwald, Morten (2018). Boosting effect of IL-7 in interferon gamma release assays to diagnose Mycobacterium tuberculosis infection. PLoS ONE, 13(8), e0202525. Public Library of Science 10.1371/journal.pone.0202525

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BACKGROUND A quarter of the world's population is estimated to be infected with Myobacterium tuberculosis (Mtb). Infection is detected by immune response to M. tuberculosis antigens using either tuberculin skin test (TST) and interferon gamma release (IGRA's), tests which have low sensitivity in immunocompromised. IL-7 is an important cytokine for T-cell function with potential to augment cytokine release in in-vitro assays. This study aimed to determine whether the addition of IL-7 in interferon-gamma release assays (IGRAs) improves its diagnostic performance of Mtb infection. METHODS 44 cases with confirmed TB and 45 household contacts without TB were recruited and 1ml of blood was stimulated in two separate IGRA's tube set: one set of standard Quantiferon TB gold tubes mitogen, TB antigen and TB Nil; one set of customized Quantiferon TB gold tubes with added IL-7. Following IFN-γ and IP-10 release was determined using ELISA. RESULTS We found that the addition of IL-7 led to significantly higher release of IFN-γ in individuals with active TB from 4.2IU/ml (IQR 1.4-6.9IU/ml) to 5.1IU/ml (IQR 1.5-8.1IU/ml, p = 0.0057), and we found an indication of a lower release of both IFN-γ and IP-10 in participants with negative tests. CONCLUSIONS In TB cases addition of IL-7 in IGRA tubes augments IFN-γ but not IP-10 release, and seems to lower the response in controls. Whether IL-7 boosted IGRA holds potential over standard IGRA needs to be confirmed in larger studies in high and low TB incidence countries.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Social and Preventive Medicine (ISPM)

UniBE Contributor:

Fenner, Lukas

Subjects:

600 Technology > 610 Medicine & health
300 Social sciences, sociology & anthropology > 360 Social problems & social services

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Tanya Karrer

Date Deposited:

12 Sep 2018 16:40

Last Modified:

24 Oct 2019 14:56

Publisher DOI:

10.1371/journal.pone.0202525

PubMed ID:

30157233

Additional Information:

Hellen Hiza and Lukas Fenner contributed equally to this work

BORIS DOI:

10.7892/boris.119735

URI:

https://boris.unibe.ch/id/eprint/119735

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