Mastitis diagnostics: quantitative PCR for Staphylococcus aureus genotype B in bulk tank milk

Boss, R.; Naskova, J.; Steiner, Adrian; Graber, H.U. (2011). Mastitis diagnostics: quantitative PCR for Staphylococcus aureus genotype B in bulk tank milk. Journal of dairy science, 94(1), pp. 128-37. Savoy, Ill.: American Dairy Science Association 10.3168/jds.2010-3251

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A novel real-time quantitative PCR assay for detecting the pathogenic and contagious Staphylococcus aureus genotype B (GTB) in bulk tank milk was developed and evaluated. The detection of this pathogen in bulk tank milk would greatly facilitate its control, as it is responsible for great economic loss in Swiss dairy herds. The assay is based on the simultaneous detection of 3 GTB-typical target sequences, including 2 enterotoxin genes and a polymorphism within the leucotoxin E gene. A variety of mastitis-associated bacteria was used to validate the assays, resulting in an analytical specificity of 100% and high repeatability. The analytical sensitivity in milk was 40 cfu/mL. An exponential association between simulated cow prevalence and quantitative PCR result was observed. An initial field study revealed 1 GTB-positive herd among the 33 studied herds. This novel assay for bulk tank milk analysis is suitable for routine purposes and is expected to be an effective tool for minimizing Staph. aureus GTB in Swiss dairy herds.

Item Type:

Journal Article (Original Article)


05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants

UniBE Contributor:

Steiner, Adrian


600 Technology > 630 Agriculture




American Dairy Science Association




Factscience Import

Date Deposited:

04 Oct 2013 14:31

Last Modified:

13 Mar 2020 15:51

Publisher DOI:


Web of Science ID:


URI: (FactScience: 218259)

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