How high-resolution techniques enable reliable steroid identification and quantification

Bileck, Andrea; Flück Pandey, Christa Emma; Dhayat, Nasser; Grössl, Michael (2018). How high-resolution techniques enable reliable steroid identification and quantification (In Press). Journal of steroid biochemistry and molecular biology Elsevier 10.1016/j.jsbmb.2018.09.018

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Due to possible matrix interferences and artefact generation during sample preparation, careful method validation is required for quantitative bioanalytical methods, especially for analytes that are only present in low concentrations. Using the identification and quantification of progesterone metabolite in the urine of newborns as an example, we show how modern high-resolution instruments can be used to verify analyte assignment and avoid pitfalls commonly encountered by the use of low-resolution instruments.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Nephrology and Hypertension
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Nephrologie / Hypertonie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Endokrinologie / Diabetologie / Metabolik (Pädiatrie)
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE)
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine

UniBE Contributor:

Bileck, Andrea; Flück Pandey, Christa Emma; Dhayat, Nasser and Grössl, Michael

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0960-0760

Publisher:

Elsevier

Language:

English

Submitter:

Nasser Dhayat

Date Deposited:

01 Oct 2018 14:56

Last Modified:

02 Oct 2018 01:32

Publisher DOI:

10.1016/j.jsbmb.2018.09.018

PubMed ID:

30268410

BORIS DOI:

10.7892/boris.119972

URI:

https://boris.unibe.ch/id/eprint/119972

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