Distinct proteinase K-resistant prion protein fragment in goats with no signs of disease in a classical scrapie outbreak

Bouzalas, I.G.; Lortscher, F.; Dovas, I.; Oevermann, Anna; Langeveld, J.P.; Papanastassopoulou, M.; Papadopoulos, O.; Zurbriggen, Andreas; Seuberlich, Torsten (2011). Distinct proteinase K-resistant prion protein fragment in goats with no signs of disease in a classical scrapie outbreak. Journal of clinical microbiology, 49(6), pp. 2109-15. Washington, D.C.: American Society for Microbiology 10.1128/JCM.02033-10

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Considerable efforts have been directed toward the identification of small-ruminant prion diseases, i.e., classical and atypical scrapie as well as bovine spongiform encephalopathy (BSE). Here we report the in-depth molecular analysis of the proteinase K-resistant prion protein core fragment (PrP(res)) in a highly scrapie-affected goat flock in Greece. The PrP(res) profile by Western immunoblotting in most animals was that of classical scrapie in sheep. However, in a series of clinically healthy goats we identified a unique C- and N-terminally truncated PrP(res) fragment, which is akin but not identical to that observed for atypical scrapie. These findings reveal novel aspects of the nature and diversity of the molecular PrP(res) phenotypes in goats and suggest that these animals display a previously unrecognized prion protein disorder.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > NeuroCenter
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Oevermann, Anna; Zurbriggen, Andreas and Seuberlich, Torsten

ISSN:

0095-1137

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:31

Last Modified:

06 Jun 2018 10:06

Publisher DOI:

10.1128/JCM.02033-10

Web of Science ID:

000291024500006

URI:

https://boris.unibe.ch/id/eprint/11998 (FactScience: 218268)

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