Isolation and genotyping of Toxoplasma gondii causing fatal systemic toxoplasmosis in an immunocompetent 10-year-old cat

Spycher, A.; Geigy, C.; Howard, J.; Posthaus, Horst; Gendron, Karine; Gottstein, Bruno; Debache, K.; Herrmann, D.C.; Schares, G.; Frey, Caroline (2011). Isolation and genotyping of Toxoplasma gondii causing fatal systemic toxoplasmosis in an immunocompetent 10-year-old cat. Journal of veterinary diagnostic investigation, 23(1), pp. 104-8. Columbia, Mo.: American Association of Veterinary Laboratory Diagnosticians AAVLD 10.1177/104063871102300117

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A 10-year-old male, neutered domestic shorthair cat was presented with fever, anorexia, vomiting, and diarrhea. Serologic testing for Feline immunodeficiency virus and Feline leukemia virus were negative. Fine-needle aspirates of mesenteric lymph nodes revealed the presence of banana-shaped apicomplexan parasites. The cat died after 4 days of hospitalization. Postmortem polymerase chain reaction (PCR) analysis confirmed the presence of Toxoplasma gondii in all examined organs. Parasites were ex vivo isolated in outbred mice and subsequently transferred into cell culture. Genotyping, using genetic markers for SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico for PCR-restriction fragment length polymorphism, revealed infection with type II T. gondii displaying type II alleles at all loci except Apico, which exhibited a type I allele. This is the most frequently identified genotype among cats acting as definitive hosts in central Europe, but to the authors' knowledge, it has never been associated with systemic toxoplasmosis in an adult, immunocompetent cat.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic > Small Animal Clinic, Internal Medicine
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Clinical Radiology
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Central Clinical Laboratory
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Spycher, Andrea; Geigy, Caroline; Howard, Judith; Posthaus, Horst; Gendron, Karine; Gottstein, Bruno; Debache, Karim and Frey, Caroline

Subjects:

600 Technology > 630 Agriculture

ISSN:

1040-6387

Publisher:

American Association of Veterinary Laboratory Diagnosticians AAVLD

Language:

English

Submitter:

Judith Howard

Date Deposited:

04 Oct 2013 14:31

Last Modified:

25 Jan 2016 15:50

Publisher DOI:

10.1177/104063871102300117

PubMed ID:

21217037

BORIS DOI:

10.7892/boris.12043

URI:

https://boris.unibe.ch/id/eprint/12043 (FactScience: 218317)

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