Microbial and host immune factors as drivers of COPD.

Mika, Moana; Nita, Izabela Magdalena; Morf, Laura Ursina; Qi, Weihong; Beyeler, Seraina Martina; Bernasconi, Eric; Marsland, Benjamin J; Ott, Sebastian Robert; von Garnier, Christophe; Hilty, Markus (2018). Microbial and host immune factors as drivers of COPD. ERJ Open Research, 4(3) European Respiratory Society 10.1183/23120541.00015-2018

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Compartmentalisation of the respiratory tract microbiota in patients with different chronic obstructive pulmonary disease (COPD) severity degrees needs to be systematically investigated. In addition, it is unknown if the inflammatory and emphysematous milieux in patients with COPD are associated with changes in the respiratory tract microbiota and host macrophage gene expression. We performed a cross-sectional study to compare non-COPD controls (n=10) to COPD patients (n=32) with different disease severity degrees. Samples (n=187) were obtained from different sites of the upper and lower respiratory tract. Microbiota analyses were performed by 16S ribosomal RNA gene sequencing and host gene expression analyses by quantitative real-time PCR of distinct markers of bronchoalveolar lavage cells. Overall, the microbial communities of severe COPD (Global Initiative for Chronic Obstructive Lung Disease (GOLD) grade 3/4) patients clustered significantly differently to controls and less severe COPD (GOLD 1/2) patients (permutational multivariate ANOVA (MANOVA), p=0.001). However, we could not detect significant associations between the different sampling sites in the lower airways. In addition, the chosen set of host gene expression markers significantly separated COPD GOLD 3/4 patients, and we found correlations between the composition of the microbiota and the host data. In conclusion, this study demonstrates associations between host gene expression and microbiota profiles that may influence the course of COPD.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Mika, Moana; Nita, Izabela Magdalena; Morf, Laura Ursina; Beyeler, Seraina Martina; Ott, Sebastian Robert; von Garnier, Christophe and Hilty, Markus

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

2312-0541

Publisher:

European Respiratory Society

Language:

English

Submitter:

Markus Hilty

Date Deposited:

22 Oct 2018 09:21

Last Modified:

15 Jan 2019 15:26

Publisher DOI:

10.1183/23120541.00015-2018

PubMed ID:

29992131

BORIS DOI:

10.7892/boris.120528

URI:

https://boris.unibe.ch/id/eprint/120528

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