3D artificial round section micro-vessels to investigate endothelial cells under physiological flow conditions

Sfriso, Riccardo; Zhang, Shengye; Bichsel, Colette Andrea; Steck, Oliver Thomas; Despont, Alain; Guenat, Olivier Thierry; Rieben, Robert (2018). 3D artificial round section micro-vessels to investigate endothelial cells under physiological flow conditions. Scientific Reports, 8(1), p. 5898. Nature Publishing Group 10.1038/s41598-018-24273-7

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In the context of xenotransplantation, in ischemia/reperfusion injury as well as in cardiovascular research, the study of the fascinating interplay between endothelial cells (EC) and the plasma cascade systems often requires in vitro models. Blood vessels are hardly reproducible with standard flat-bed culture systems and flow-plate assays are limited in their low surface-to-volume ratio which impedes the study of the anticoagulant properties of the endothelial cells. According to the 3R regulations (reduce, replace and refine animal experimentation) we developed a closed circuit microfluidic in vitro system in which endothelial cells are cultured in 3D round section microchannels and subjected to physiological, pulsatile flow. In this study, a 3D monolayer of porcine aortic EC was perfused with human serum to mimic a xenotransplantation setting. Complement as well as EC activation was assessed in the presence or absence of complement inhibitors showing the versatility of the model for drug testing. Complement activation products as well as E-selectin expression were detected and visualized in situ by high resolution confocal microscopy. Furthermore, porcine pro-inflammatory cytokines as well as soluble complement components in the recirculating fluid phase were detected after human serum perfusion providing a better overview of the artificial vascular environment.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Herz und Gefässe
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50
10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Lung Regeneration Technologies
09 Interdisciplinary Units > Microscopy Imaging Center MIC

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Sfriso, Riccardo; Zhang, Shengye; Steck, Oliver Thomas; Despont, Alain; Guenat, Olivier Thierry and Rieben, Robert

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

2045-2322

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Riccardo Sfriso

Date Deposited:

29 Oct 2018 16:43

Last Modified:

12 Jun 2019 08:53

Publisher DOI:

10.1038/s41598-018-24273-7

PubMed ID:

29651108

BORIS DOI:

10.7892/boris.120726

URI:

https://boris.unibe.ch/id/eprint/120726

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