A Zebrafish Model for Infection with the Obligate Intracellular Pathogen .

Fehr, Alexander G J; Ruetten, Maja; Seth-Smith, Helena M B; Nufer, Lisbeth; Vögtlin, Andrea; Lehner, Angelika; Greub, Gilbert; Crosier, Philip S; Neuhauss, Stephan C F; Vaughan, Lloyd (2016). A Zebrafish Model for Infection with the Obligate Intracellular Pathogen . Frontiers in Microbiology, 7, p. 1829. Frontiers 10.3389/fmicb.2016.01829

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Obligate intracellular chlamydial bacteria of the Planctomycetes-Verrucomicrobia-Chlamydiae (PVC) superphylum are important pathogens of terrestrial and marine vertebrates, yet many features of their pathogenesis and host specificity are still unknown. This is particularly true for families such as the which, in addition to epithelia, cellular targets for nearly all , can infect and replicate in macrophages, an important arm of the innate immune system or in their free-living amoebal counterparts. An ideal pathogen model system should include both host and pathogen, which led us to develop the first larval zebrafish model for chlamydial infections with . By varying the means and sites of application, epithelial cells of the swim bladder, endothelial cells of the vasculature and phagocytosing cells of the innate immune system became preferred targets for infection in zebrafish larvae. Through the use of transgenic zebrafish, we could observe recruitment of neutrophils to the infection site and demonstrate for the first time that is taken up and replicates in these phagocytic cells and not only in macrophages. Furthermore, we present evidence that myeloid differentiation factor 88 (MyD88) mediated signaling plays a role in the innate immune reaction to , eventually by Toll-like receptor (TLRs) recognition. Infected larvae with depleted levels of MyD88 showed a higher infection load and a lower survival rate compared to control fish. This work presents a new and potentially powerful non-mammalian experimental model to study the pathology of chlamydial virulence and opens up new possibilities for investigation of other members of the PVC superphylum.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Vögtlin, Andrea

Subjects:

600 Technology > 630 Agriculture

ISSN:

1664-302X

Publisher:

Frontiers

Language:

English

Submitter:

Monika Mumenthaler

Date Deposited:

01 Nov 2018 15:15

Last Modified:

04 Nov 2018 02:32

Publisher DOI:

10.3389/fmicb.2016.01829

PubMed ID:

27917158

Uncontrolled Keywords:

Chlamydia MyD88 PVC superphylum Waddlia endothelial cells neutrophils swim bladder infection zebrafish

BORIS DOI:

10.7892/boris.120822

URI:

https://boris.unibe.ch/id/eprint/120822

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