The integrase of the Macrococcus caseolyticus resistance island mecD (McRI ) inserts DNA site-specifically into Staphylococcus and Bacillus chromosomes.

Schwendener, Sybille; Perreten, Vincent (2018). The integrase of the Macrococcus caseolyticus resistance island mecD (McRI ) inserts DNA site-specifically into Staphylococcus and Bacillus chromosomes. Molecular microbiology, 110(3), pp. 455-468. Wiley 10.1111/mmi.14112

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The methicillin resistance gene mecD has been recently identified on chromosomal islands in Macrococcus caseolyticus (McRI ). The 5' end of McRI carries an integrase (int) of the tyrosine recombinase family and two genes (intR and xis) encoding putative DNA-binding proteins. The islands are integrated site-specifically at the 3' end of the rpsI gene, a highly conserved locus in Gram-positive bacteria. Moreover, the rpsI gene of some Staphylococcus and Bacillus strains was found to be followed by a related integrase, raising the question of whether McRI could be transferred to these species. We used circular model elements carrying 5' end fragments of McRI -1 to demonstrate that the int enzyme and the attachment (att) site were sufficient to mediate site-specific DNA integration into the rpsI locus of Staphylococcus aureus, Staphylococcus pseudintermedius and Bacillus thuringiensis in vivo. Including xis in the model element stimulated both integrative and excisive recombination reactions and influenced the Int enzyme in att site selection. The intR gene functions as a negative regulator of int and xis. The int-xis genes of McRI -1 encode a site-specific recombination function that enables the acquisition of McRI in new hosts and the potential dissemination of broad-spectrum β-lactam resistance across genus barriers.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology > Molecular Bacterial Epidemiology and Infectiology
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Schwendener, Sybille and Perreten, Vincent

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0950-382X

Publisher:

Wiley

Language:

English

Submitter:

Vincent Perreten

Date Deposited:

06 Nov 2018 10:30

Last Modified:

06 Nov 2018 11:10

Publisher DOI:

10.1111/mmi.14112

PubMed ID:

30152907

BORIS DOI:

10.7892/boris.120937

URI:

https://boris.unibe.ch/id/eprint/120937

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