Predominance of a macrolide-lincosamide-resistant Brachyspira hyodysenteriae of sequence type 196 in Swiss pig herds.

Garcia Martin, Ana Bélen; Perreten, Vincent; Rossano, Alexandra; Schmitt, Sarah; Nathues, Heiko; Zeeh, Friederike (2018). Predominance of a macrolide-lincosamide-resistant Brachyspira hyodysenteriae of sequence type 196 in Swiss pig herds. Veterinary microbiology, 226, pp. 97-102. Elsevier 10.1016/j.vetmic.2018.10.007

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Worldwide emergence of antimicrobial-resistant Brachyspira (B.) hyodysenteriae led us question whether specific clones are present in Switzerland. Fifty-one B. hyodysenteriae isolates originating from 27 different Swiss pig herds sampled between 2010 and 2017 were characterised. Multilocus sequence typing revealed the presence of four different sequence types (STs) ST6, ST66, ST196 and ST197 with ST196 being predominant. Antimicrobial susceptibility to six different antimicrobial agents was determined by measurement of the minimal inhibitory concentration by broth dilution. Isolates were examined for the presence of point mutations and genes known to be associated with antimicrobial resistance in B. hyodysenteriae by PCR and sequence analysis. Forty-one isolates belonging to ST6 (n = 1), ST66 (n = 4) and ST196 (n = 36) exhibited decreased susceptibility to macrolides and lincomycin associated with an A2058 T/G mutation in the 23S rRNA gene. One isolate of ST66 and five isolates of ST196 exhibited decreased susceptibility to doxycycline associated with a G1058C mutation in the 16S rRNA gene. The Swiss B. hyodysenteriae population is characterised by a low genetic diversity, with macrolide-lincosamide-resistant isolates of ST196 being predominant.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology > Molecular Bacterial Epidemiology and Infectiology
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Swine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Garcia Martin, Ana Bélen; Perreten, Vincent; Rossano, Alexandra; Nathues, Heiko and Zeeh, Friederike

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0378-1135

Publisher:

Elsevier

Language:

English

Submitter:

Vincent Perreten

Date Deposited:

09 Nov 2018 09:36

Last Modified:

24 Oct 2019 12:29

Publisher DOI:

10.1016/j.vetmic.2018.10.007

PubMed ID:

30389050

Uncontrolled Keywords:

Antibiotic resistance Antimicrobial susceptibility Genotyping Minimal inhibitory concentration Swine dysentery

BORIS DOI:

10.7892/boris.121051

URI:

https://boris.unibe.ch/id/eprint/121051

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