Generation of an equine biobank to be used for Functional Annotation of Animal Genomes project.

Burns, E N; Bordbari, M H; Mienaltowski, M J; Affolter, V K; Barro, M V; Gianino, F; Gianino, G; Giulotto, E; Kalbfleisch, T S; Katzman, S A; Lassaline, M; Leeb, Tosso; Mack, M; Müller, E. J.; MacLeod, J N; Ming-Whitfield, B; Alanis, C R; Raudsepp, T; Scott, E; Vig, S; ... (2018). Generation of an equine biobank to be used for Functional Annotation of Animal Genomes project. Animal genetics, 49(6), pp. 564-570. Wiley 10.1111/age.12717

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The Functional Annotation of Animal Genomes (FAANG) project aims to identify genomic regulatory elements in both sexes across multiple stages of development in domesticated animals. This study represents the first stage of the FAANG project for the horse, Equus caballus. A biobank of 80 tissue samples, two cell lines and six body fluids was created from two adult Thoroughbred mares. Ante-mortem assessments included full physical examinations, lameness, ophthalmologic and neurologic evaluations. Complete blood counts and serum biochemistries were also performed. At necropsy, in addition to tissue samples, aliquots of serum, ethylenediaminetetraacetic acid (EDTA) plasma, heparinized plasma, cerebrospinal fluid, synovial fluid, urine and microbiome samples from all regions of the gastrointestinal and urogenital tracts were collected. Epidermal keratinocytes and dermal fibroblasts were cultured from skin samples. All tissues were grossly and histologically evaluated by a board-certified veterinary pathologist. The results of the clinical and pathological evaluations identified subclinical eosinophilic and lymphocytic infiltration throughout the length of the gastrointestinal tract as well as a mild clinical lameness in both animals. Each sample was cryo-preserved in multiple ways, and nuclei were extracted from selected tissues. These samples represent the first published systemically healthy equine-specific biobank with extensive clinical phenotyping ante- and post-mortem. The tissues in the biobank are intended for community-wide use in the functional annotation of the equine genome. The use of the biobank will improve the quality of the reference annotation and allow all equine researchers to elucidate unknown genomic and epigenomic causes of disease.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Dermatology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pathologie > Forschungsgruppe Dermatologie
05 Veterinary Medicine > Research Foci > DermFocus
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Leeb, Tosso and Müller, Eliane Jasmine

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

0268-9146

Publisher:

Wiley

Language:

English

Submitter:

Tosso Leeb

Date Deposited:

12 Nov 2018 11:03

Last Modified:

25 Oct 2019 02:47

Publisher DOI:

10.1111/age.12717

PubMed ID:

30311254

Uncontrolled Keywords:

biorepository genome regulation horse necropsy nuclei isolation tissue collection

BORIS DOI:

10.7892/boris.121083

URI:

https://boris.unibe.ch/id/eprint/121083

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