Vacirca, D.; Perdicchio, M.; Campisi, E.; Delunardo, F.; Ortona, E.; Margutti, P.; Teggi, A.; Gottstein, B.; Siracusano, A. (2011). Favourable prognostic value of antibodies anti-HSP20 in patients with cystic echinococcosis: a differential immunoproteomic approach. Parasite immunology, 33(3), pp. 193-8. Oxford: Blackwell Scientific Publications 10.1111/j.1365-3024.2010.01264.x
Full text not available from this repository. (Request a copy)Seeking biomarkers reflecting disease development in cystic echinococcosis (CE), we used a proteomic approach linked to immunological characterisation for the identification of respective antigens. Two-dimensional gel electrophoresis (2-DE) of sheep hydatid fluid, followed by immunoblot analysis (IB) with sera from patients with distinct phases of disease, enabled us to identify by mass spectrometry heat shock protein 20 (HSP20) as a potential marker of active CE. Using IB, antibodies specific to the 34 kDa band of HSP20 were detected in sera from 61/95 (64%) patients with CE, but not in sera from healthy subjects. IB revealed anti-HSP20 antibodies in a higher percentage of sera from patients with active disease than in sera from patients with inactive disease (81 vs. 24%; P = 10(-4)). These primary results were confirmed in a long-term follow-up study after pharmacological and surgical treatment. Herewith anti-HSP20 antibody levels significantly decreased over the course of treatment in sera from patients with cured disease, relative to sera from patients with progressive disease (P = 0.017). Thus, during CE, a comprehensive strategy of proteomic identification combined with immunological validation represents a promising approach for the identification of biomarkers useful for the prognostic assessment of treatment of CE patients.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology |
UniBE Contributor: |
Gottstein, Bruno |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0141-9838 |
Publisher: |
Blackwell Scientific Publications |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:31 |
Last Modified: |
05 Dec 2022 14:10 |
Publisher DOI: |
10.1111/j.1365-3024.2010.01264.x |
PubMed ID: |
21306401 |
URI: |
https://boris.unibe.ch/id/eprint/12118 (FactScience: 218401) |
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