Comparison of Profilometric and Microindentation Analyses for Determining the Impact of Saliva on the Abrasion of Initially Eroded Enamel.

Steiger-Ronay, Valerie; Tektas, Sibel; Attin, Thomas; Lussi, Adrian; Becker, Klaus; Wiedemeier, Daniel B; Beyeler, Barbara; Saads Carvalho, Thiago (2019). Comparison of Profilometric and Microindentation Analyses for Determining the Impact of Saliva on the Abrasion of Initially Eroded Enamel. Caries research, 53(1), pp. 33-40. Karger 10.1159/000489133

[img] Text
CariesRes_Paper.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (383kB) | Request a copy

The aim of this in vitro study was to investigate the impact of saliva on the abrasion of eroded enamel using two measuring methods. A total of 80 bovine enamel specimens from 20 bovine incisors were allocated to four experimental groups (n = 20 specimens per group). After baseline surface microhardness (SMH) measurements and profilometry all specimens were subjected to erosion (2 min, 1% citric acid, pH: 3.6, 37°C). SMH was determined again, and the depths of the Knoop indentations were calculated. Thereafter, specimens were incubated in human saliva (group 1 - no incubation/control, group 2 - 0.5 h, group 3 - 1 h, group 4 - 2 h) before toothbrush abrasion was performed. After final SMH measurements and profilometry, indentations were remeasured, and surface loss was calculated. SMH did not return to baseline values regardless of the length of saliva incubation. Further, an irreversible substance loss was observed for all specimens. With the indentation method, significantly (p < 0.05) more substance loss was found for controls (least square means ± standard error of 198 ± 19 nm) than for groups 2-4 (110 ± 10, 114 ± 11, and 105 ± 14 nm). Profilometric assessment showed significantly more substance loss for controls (122 ± 8 nm) than for group 4 (106 ± 5 nm). Intraclass correlation for interrater reliability between measurement methods was low (0.21, CI: 0.1-0.3), indicating poor agreement. Exposure of eroded enamel to saliva for up to 2 h could not re-establish the original SMH. The amount of measured substance loss depended on the measurement method applied.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Saads Carvalho, Thiago


600 Technology > 610 Medicine & health








Thiago Saads Carvalho

Date Deposited:

08 Apr 2019 16:33

Last Modified:

08 Apr 2019 16:33

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Abrasion Erosion Profilometry Saliva Surface microhardness measurement Tooth brushing




Actions (login required)

Edit item Edit item
Provide Feedback