A comprehensive urinary steroid analysis strategy using two-dimensional gas chromatography - time of flight mass spectrometry.

Bileck, Andrea; Verouti, Sophia N; Escher, Geneviève; Vogt, Bruno; Grössl, Michael (2018). A comprehensive urinary steroid analysis strategy using two-dimensional gas chromatography - time of flight mass spectrometry. Analyst, 143(18), pp. 4484-4494. Royal Society of Chemistry 10.1039/c7an01990d

[img] Text
c7an01990d.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (2MB) | Request a copy

Steroids are key players in a high variety of physiological processes and are typically analyzed for the diagnosis of hormonal disorders. Due to their chemical and structural similarity many of these metabolites cannot be separated by conventional techniques such as liquid chromatography. Herein, we present an analysis strategy based on two dimensional gas chromatography (GC×GC) coupled to time-of-flight mass spectrometry (TOF MS) which demonstrates superior separation power and enables comprehensive screening of steroids. We show absolute quantitation of 40 steroids in human urine over three orders of magnitude with limits of detection ≤50 nM and the tentative identification of additional 30 steroids based on accurate mass, isotopic pattern analysis and spectral similarity matching to known steroids. The method displays excellent inter- and intra-day stability, repeatability and recovery and was validated for clinical routine analysis. Additionally, we demonstrate the potential of the approach for untargeted analysis of urinary steroids in mouse and rat.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Nephrology and Hypertension

UniBE Contributor:

Bileck, Andrea; Escher, Geneviève; Vogt, Bruno and Grössl, Michael

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1364-5528

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Geneviève Escher

Date Deposited:

17 Sep 2019 12:21

Last Modified:

29 Oct 2019 07:02

Publisher DOI:

10.1039/c7an01990d

PubMed ID:

30156584

BORIS DOI:

10.7892/boris.122226

URI:

https://boris.unibe.ch/id/eprint/122226

Actions (login required)

Edit item Edit item
Provide Feedback