Stem cell secretome attenuates acute rejection in rat lung allotransplant.

Pieróg, Jaroslaw; Fytianos, Kleanthis; Tamò, Luca Giuseppe Athos; Simillion, Cedric André Marie; Taddeo, Adriano; Kocher, Gregor; Gugger, Mathias; Grodzki, Tomasz; Heller, Manfred; Blank, Fabian; Geiser, Thomas; Schmid, Ralph; Gazdhar, Amiq (2018). Stem cell secretome attenuates acute rejection in rat lung allotransplant. (In Press). Interactive cardiovascular and thoracic surgery Oxford University Press 10.1093/icvts/ivy306

[img] Text
ivy306.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (808kB) | Request a copy

OBJECTIVES Stem cells secrete significant amounts of bioactive factors in their secretome that can be immunosuppressive. We studied the effect of the secretome obtained from bone marrow-derived mesenchymal stem cells (BMSC-sec) in combination with cyclosporine A following acute rejection of lung allografts in the rat. METHODS Lung allotransplants were performed from male Brown Norway donor rats to recipient male Fisher 344 rats. Rat BMSC-sec was introduced intratracheally in the recipient every day after the transplant until the day the animal was sacrificed. Group A (n = 5) received control medium and cyclosporine A (2.5 mg/kg body weight intraperitoneally) for 5 days post-transplant and group B (n = 5) received BMSC-sec and cyclosporine A. Blood gas analysis was performed to assess graft function at day 5 only from the graft, and the tissue was sampled for measurement of the wet/dry ratio and histological grading of rejection. RESULTS All control animals (group A) showed severe signs of rejection. At day 5 grafts in group B showed improved gas exchange (i.e. mean PaO2 mmHg 237.9 ± 130 mmHg vs 24.9 ± 7.8 mmHg in group A). Histological examination according to the International Society of Heart and Lung Transplantation (ISHLT) revealed moderate to severe rejection in all animals in group A (III B) and a significant improvement in group B (I-IIA). The wet/dry ratio was also reduced in group B to 6.19 ± 0.6 compared to 9.36 ± 2 in group A. Furthermore, in vitro T-cell proliferation was reduced after treatment with BMSC-sec for CD 3 cells (69.55 ± 07 vs 73 ± 0.84), for CD 4 (24.95 ± 1.2 vs 27.75 ± 0.21) and for CD 8 cells (3.75 ± 0.2 vs 5.68 ± 0.02). CONCLUSIONS The BMSC-sec is a promising novel cell-based therapeutic option for acute rejection in a rat lung allograft model.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Thoracic Surgery
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Thoraxchirurgie
04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Plastic and Hand Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Handchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Massenspektrometrie- und Proteomics-Labor
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Protein- und Zellbiologie
09 Interdisciplinary Units > Microscopy Imaging Center MIC
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Live Cell Imaging (LCI)

UniBE Contributor:

Fytianos, Kleanthis; Tamò, Luca Giuseppe Athos; Simillion, Cedric André Marie; Taddeo, Adriano; Kocher, Gregor; Heller, Manfred; Blank, Fabian; Geiser, Thomas; Schmid, Ralph and Gazdhar, Amiq

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1569-9293

Publisher:

Oxford University Press

Language:

English

Submitter:

Marla Rittiner

Date Deposited:

07 Feb 2019 15:42

Last Modified:

19 Feb 2019 14:32

Publisher DOI:

10.1093/icvts/ivy306

PubMed ID:

30508108

BORIS DOI:

10.7892/boris.122532

URI:

https://boris.unibe.ch/id/eprint/122532

Actions (login required)

Edit item Edit item
Provide Feedback