Convergent evolution of a metabolic switch between aphid and caterpillar resistance in cereals

Li, B.; Förster, C.; Robert, Christelle. A. M.; Züst, Tobias; Hu, Lingfei; Machado, Ricardo A. R.; Berset, Jean Daniel; Handrick, V.; Knauer, T.; Hensel, G.; Chen, W.; Kumlehn, J.; Yang, P.; Keller, B.; Gershenzon, J.; Jander, G.; Köllner, T. G.; Erb, Matthias (2018). Convergent evolution of a metabolic switch between aphid and caterpillar resistance in cereals. Science Advances, 4(12), eaat6797. American Association for the Advancement of Science 10.1126/sciadv.aat6797

[img]
Preview
Text
2018_SciAdv_eaat6797.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (1MB) | Preview

Tailoring defense responses to different attackers is important for plant performance. Plants can use secondary metabolites with dual functions in resistance and defense signaling to mount herbivore-specific responses. To date, the specificity and evolution of this mechanism are unclear. Here, we studied the functional architecture, specificity, and genetic basis of defense regulation by benzoxazinoids in cereals. We document that DIMBOA-Glc induces callose as an aphid resistance factor in wheat. O-methylation of DIMBOA-Glc to HDMBOA-Glc increases plant resistance to caterpillars but reduces callose inducibility and resistance to aphids. DIMBOA-Glc induces callose in wheat and maize, but not in Arabidopsis, while the glucosinolate 4MO-I3M does the opposite. We identify a wheat O-methyltransferase (TaBX10) that is induced by caterpillar feeding and converts DIMBOA-Glc to HDMBOA-Glc in vitro. While the core pathway of benzoxazinoid biosynthesis is conserved between wheat and maize, the wheat genome does not contain close homologs of the maize DIMBOA-Glc O-methyltransferase genes, and TaBx10 is only distantly related. Thus, the functional architecture of herbivore-specific defense regulation is similar in maize and wheat, but the regulating biosynthetic genes likely evolved separately. This study shows how two different cereal species independently achieved herbivore-specific defense activation by regulating secondary metabolite production.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Biotic Interactions
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Robert, Christelle Aurélie Maud; Züst, Tobias; Hu, Lingfei; Ruiz Machado, Ricardo Alberto; Berset, Jean Daniel and Erb, Matthias

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

2375-2548

Publisher:

American Association for the Advancement of Science

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

31 Jan 2019 09:02

Last Modified:

31 Oct 2019 14:51

Publisher DOI:

10.1126/sciadv.aat6797

PubMed ID:

30525102

BORIS DOI:

10.7892/boris.122687

URI:

https://boris.unibe.ch/id/eprint/122687

Actions (login required)

Edit item Edit item
Provide Feedback