Novel peptide-dendrimer/lipid/oligonucleotide ternary complexes for efficient cellular uptake and improved splice-switching activity

Saher, Osama; Rocha, Cristina S.J.; Zaghloul, Eman M.; Wiklander, Oscar P.B.; Zamolo, Susanna; Heitz, Marc; Ezzat, Kariem; Gupta, Dhanu; Reymond, Jean-Louis; Zain, Rula; Hollfelder, Florian; Darbre, Tamis; Lundin, Karin E.; EL Andaloussi, Samir; Smith, C.I. Edvard (2018). Novel peptide-dendrimer/lipid/oligonucleotide ternary complexes for efficient cellular uptake and improved splice-switching activity. European journal of pharmaceutics and biopharmaceutics, 132, pp. 29-40. Elsevier 10.1016/j.ejpb.2018.09.002

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Despite the advances in gene therapy and in oligonucleotide (ON) chemistry, efficient cellular delivery remains an obstacle. Most current transfection reagents suffer from low efficacy or high cytotoxicity. In this report, we describe the synergism between lipid and dendrimer delivery vectors to enhance the transfection efficiency, while avoiding high toxicity. We screened a library of 20 peptide dendrimers representing three different generations and evaluated their capability to deliver a single-stranded splice-switching ON after formulating with lipids (DOTMA/DOPE). The transfection efficiency was analyzed in 5 reporter cell lines, in serum-free and serum conditions, and with 5 different formulation protocols. All formulations displayed low cytotoxicity to the majority of the tested cell lines. The complex sizes were < 200 nm; particle size distributions of effective mixtures were < 80 nm; and, the zeta potential was dependent on the formulation buffer used. The best dendrimer enhanced transfection in a HeLa reporter cell line by 30-fold compared to untreated cells under serum-free conditions. Interestingly, addition of sucrose to the formulation enabled – for the first time – peptide dendrimers/lipid complexes to efficiently deliver splice-switching ON in the presence of serum, reaching 40-fold increase in splice switching. Finally, in vivo studies highlighted the potential of these formulae to change the biodistribution pattern to be more towards the liver (90% of injected dose) compared to the kidneys (5% of injected dose) or to unformulated ON. This success encourages further development of peptide dendrimer complexes active in serum and future investigation of mechanisms behind the influence of additives on transfection efficacy.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Zamolo, Susanna Joelle; Heitz, Marc; Reymond, Jean-Louis and Darbre, Tamis

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0939-6411

Publisher:

Elsevier

Language:

English

Submitter:

Sandra Tanja Zbinden Di Biase

Date Deposited:

19 Dec 2018 13:09

Last Modified:

19 Dec 2018 13:09

Publisher DOI:

10.1016/j.ejpb.2018.09.002

BORIS DOI:

10.7892/boris.122703

URI:

https://boris.unibe.ch/id/eprint/122703

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