Correlation between relative bacterial activity and lactate dehydrogenase gene expression of co-cultures in vitro

Walther, Carolin; Meyer-Lückel, Hendrik; Conrads, Georg; Esteves-Oliveira, Marcella; Henne, Karsten (2019). Correlation between relative bacterial activity and lactate dehydrogenase gene expression of co-cultures in vitro. Clinical oral investigations, 23(3), pp. 1225-1235. Springer-Verlag 10.1007/s00784-018-2547-2

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OBJECTIVES The present study aims at correlating the relative bacterial activity with the H concentration and the ldh expression of caries-associated bacteria in co-cultures. MATERIALS AND METHODS Well plates were prepared with BHI medium and cultures of Lactobacillus paracasei and Fusobacterium nucleatum. Bacterial growth at 37 °C was measured using a microplate-photometer before and after adding sucrose to the samples. Samples of co-cultures (n = 12) and single-species cultures (n = 3) were taken and pH was assessed. Real-time quantitative PCRs were applied targeting the 16S-gene, the 16S-rRNA, the ldh-gene, and the ldh-mRNA. RESULTS For L. paracasei with sucrose, an increase in relative bacterial activity (62.8% ± 23.5% [mean, SE]) was observed, while F. nucleatum showed a clear decrease in relative bacterial activity (- 35.0% ± 9.6%). Simultaneously, the H concentration increased (1.15E-05 mol*l-1 ± 4.61E-07 mol*l-1). Consequently, a significant positive correlation was found between L. paracasei's relative bacterial activity and H concentration (Spearman rank correlation, r = 0.638; p = 0.002), while F. nucleatum exhibited a negative correlation (r = - 0.741; p ≤ 0.001). Furthermore L. paracasei with sucrose showed a moderate, but significant positive correlation between relative bacterial activity and ldh-expression (r = 0.307; p = 0.024). CONCLUSIONS AND CLINICAL RELEVANCE The relative bacterial activity after sucrose pulse showed a significant correlation not only to the acid production (H concentration) but also to ldh expression of L. paracasei. However, further research is required to confirm these findings in a mature biofilm in vivo.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Meyer-Lückel, Hendrik


600 Technology > 610 Medicine & health








Hendrik Meyer-Lückel

Date Deposited:

21 Feb 2019 15:46

Last Modified:

23 Oct 2019 17:17

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

16S rRNA Biofilm Dental caries/etiology Fusobacteria Lactobacilli Relative bacterial activity ldh-expression




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