Correlation between relative bacterial activity and lactate dehydrogenase gene expression of co-cultures in vitro

Walther, Carolin; Meyer-Lückel, Hendrik; Conrads, Georg; Esteves-Oliveira, Marcella; Henne, Karsten (2019). Correlation between relative bacterial activity and lactate dehydrogenase gene expression of co-cultures in vitro. Clinical oral investigations, 23(3), pp. 1225-1235. Springer-Verlag 10.1007/s00784-018-2547-2

[img] Text
Walther2018_Article_CorrelationBetweenRelativeBact.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

OBJECTIVES The present study aims at correlating the relative bacterial activity with the H concentration and the ldh expression of caries-associated bacteria in co-cultures. MATERIALS AND METHODS Well plates were prepared with BHI medium and cultures of Lactobacillus paracasei and Fusobacterium nucleatum. Bacterial growth at 37 °C was measured using a microplate-photometer before and after adding sucrose to the samples. Samples of co-cultures (n = 12) and single-species cultures (n = 3) were taken and pH was assessed. Real-time quantitative PCRs were applied targeting the 16S-gene, the 16S-rRNA, the ldh-gene, and the ldh-mRNA. RESULTS For L. paracasei with sucrose, an increase in relative bacterial activity (62.8% ± 23.5% [mean, SE]) was observed, while F. nucleatum showed a clear decrease in relative bacterial activity (- 35.0% ± 9.6%). Simultaneously, the H concentration increased (1.15E-05 mol*l-1 ± 4.61E-07 mol*l-1). Consequently, a significant positive correlation was found between L. paracasei's relative bacterial activity and H concentration (Spearman rank correlation, r = 0.638; p = 0.002), while F. nucleatum exhibited a negative correlation (r = - 0.741; p ≤ 0.001). Furthermore L. paracasei with sucrose showed a moderate, but significant positive correlation between relative bacterial activity and ldh-expression (r = 0.307; p = 0.024). CONCLUSIONS AND CLINICAL RELEVANCE The relative bacterial activity after sucrose pulse showed a significant correlation not only to the acid production (H concentration) but also to ldh expression of L. paracasei. However, further research is required to confirm these findings in a mature biofilm in vivo.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Meyer-Lückel, Hendrik

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1432-6981

Publisher:

Springer-Verlag

Language:

English

Submitter:

Hendrik Meyer-Lückel

Date Deposited:

21 Feb 2019 15:46

Last Modified:

11 Apr 2019 01:31

Publisher DOI:

10.1007/s00784-018-2547-2

PubMed ID:

29980934

Uncontrolled Keywords:

16S rRNA Biofilm Dental caries/etiology Fusobacteria Lactobacilli Relative bacterial activity ldh-expression

BORIS DOI:

10.7892/boris.122908

URI:

https://boris.unibe.ch/id/eprint/122908

Actions (login required)

Edit item Edit item
Provide Feedback