Influence of highly concentrated fluoride dentifrices on remineralization characteristics of enamel in vitro

Wierichs, R J; Westphal, S; Lausch, J; Meyer-Lückel, Hendrik; Esteves-Oliveira, M (2018). Influence of highly concentrated fluoride dentifrices on remineralization characteristics of enamel in vitro. Clinical oral investigations, 22(6), pp. 2325-2334. Springer-Verlag 10.1007/s00784-018-2333-1

[img] Text
Wierichs2018_Article_InfluenceOfHighlyConcentratedF.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (651kB) | Request a copy

OBJECTIVES The aim of this in vitro study was to evaluate the role of highly fluoridated dentifrice on remineralization characteristics of lowly and highly pre-demineralized enamel artificial caries lesions. METHODS Bovine enamel specimens were prepared (pH 4.95; 21 days) and discriminated in either lowly [L] or highly [H] pre-demineralized artificial caries lesions. Specimens with a mean ΔZ (95% CI) of 5120 (4995; 5245) vol.% × μm and a mean ΔZ of 8187 (8036; 8339) vol.% × μm were selected and randomly allocated to 12 groups (n = 20). Treatments during pH-cycling (28 days; 6 × 60 min demineralization/day) were brushing 2×/day with fluoride-free (0 ppm F [L/H]), 1100 ppm F [L/H], 2800 ppm F [L/H], 5000 ppm F [L/H], 5000 ppm F + glycerin [L/H], and 5000 ppm F + TCP [L/H] containing dentifrices. Dentifrice slurries were prepared with deionized water (1:3wt/wt). After cycling specimens presenting lesion surface loss were discarded and for the remaining 202 specimens, transversal microradiographic (TMR) analyses (ΔZ/LD) were performed again. Changes in mineral loss (ΔΔZ = ΔZ - ΔZ) and lesion depth (ΔLD = LD - LD) were calculated. RESULTS Significant differences for ΔΔZ could be found between L, L, and L as well as H, H, and H/H (p ≤ 0.01; ANCOVA). Except for 0 ppm F, higher ΔΔZ could be found in highly compared with lowly demineralized specimens (p ≤ 0.004; ANCOVA). After pH-cycling, a second lesion front could only be observed in H and H. The correlation between ΔΔZ and F was moderate for lowly and highly demineralized lesions (r = 0.591; p < 0.001; r = 0.746; p < 0.001), indicating a fluoride dose response for both. CONCLUSION For both baseline substrate conditions, a dose response for fluoride could be revealed. CLINICAL SIGNIFICANCE Remineralization characteristics of enamel directly depended on baseline mineral loss.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Meyer-Lückel, Hendrik


600 Technology > 610 Medicine & health








Hendrik Meyer-Lückel

Date Deposited:

21 Feb 2019 16:02

Last Modified:

20 Dec 2019 04:54

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Enamel caries Fluoride Non-cavitated caries lesions Remineralization Toothpastes pH-cycling




Actions (login required)

Edit item Edit item
Provide Feedback