Proteomics identification of radiation-induced changes of membrane proteins in the rat model of arteriovenous malformation in pursuit of targets for brain AVM molecular therapy

Simonian, Margaret; Shirasaki, Dyna; Lee, Vivienne S.; Bervini, David; Grace, Michael; Ogorzalek Loo, Rachel R.; Loo, Joseph A.; Molloy, Mark P.; Stoodley, Marcus A. (2018). Proteomics identification of radiation-induced changes of membrane proteins in the rat model of arteriovenous malformation in pursuit of targets for brain AVM molecular therapy. Clinical proteomics, 15(43), p. 43. BioMed Central 10.1186/s12014-018-9217-x

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Background

Rapid identification of novel targets and advancement of a vascular targeting strategy requires a comprehensive assessment of AVM endothelial membrane protein changes in response to irradiation. The aim of this study is to provide additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery.
Methods

We employed in vivo biotinylation methodology that we developed, to label membrane proteins in the rat model of AVM post radiosurgery. Mass spectrometry expression (MSE) analysis was used to identify and quantify surface protein expression between irradiated and non irradiated rats, which mimics a radiosurgical treatment approach.
Results

Our proteomics data revealed differentially expressed membrane proteins between irradiated and non irradiated rats, e.g. profilin-1, ESM-1, ion channel proteins, annexin A2 and lumican.
Conclusion

This work provides additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurosurgery

UniBE Contributor:

Bervini, David

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1559-0275

Publisher:

BioMed Central

Language:

English

Submitter:

Nicole Söll

Date Deposited:

04 Feb 2019 19:12

Last Modified:

04 Nov 2019 08:27

Publisher DOI:

10.1186/s12014-018-9217-x

PubMed ID:

30602943

BORIS DOI:

10.7892/boris.123054

URI:

https://boris.unibe.ch/id/eprint/123054

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