Molecular characterization and prospective isolation of human fetal cochlear hair cell progenitors.

Roccio, Marta; Perny, Michael; Ealy, Megan; Widmer, Hans Ruedi; Heller, Stefan; Senn, Pascal (2018). Molecular characterization and prospective isolation of human fetal cochlear hair cell progenitors. Nature communications, 9(4027), pp. 1-14. Nature Publishing Group 10.1038/s41467-018-06334-7

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Sensory hair cells located in the organ of Corti are essential for cochlear mechanosensation. Their loss is irreversible in humans resulting in permanent hearing loss. The development of therapeutic interventions for hearing loss requires fundamental knowledge about similarities and potential differences between animal models and human development as well as the establishment of human cell based-assays. Here we analyze gene and protein expression of the developing human inner ear in a temporal window spanning from week 8 to 12 post conception, when cochlear hair cells become specified. Utilizing surface markers for the cochlear prosensory domain, namely EPCAM and CD271, we purify postmitotic hair cell progenitors that, when placed in culture in three-dimensional organoids, regain proliferative potential and eventually differentiate to hair cell-like cells in vitro. These results provide a foundation for comparative studies with otic cells generated from human pluripotent stem cells and for establishing novel platforms for drug validation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ear, Nose and Throat Disorders (ENT)
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurosurgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pavillon 52 > Forschungsgruppe Audiologie

UniBE Contributor:

Roccio, Marta; Perny, Michael; Widmer, Hans Rudolf and Senn, Pascal

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

2041-1723

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Nicole Söll

Date Deposited:

09 Jan 2019 10:42

Last Modified:

23 Oct 2019 10:10

Publisher DOI:

10.1038/s41467-018-06334-7

PubMed ID:

30279445

BORIS DOI:

10.7892/boris.123057

URI:

https://boris.unibe.ch/id/eprint/123057

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