Fibrinolytic Capacity of Desmoteplase Compared to Tissue Plasminogen Activator in Rabbit Eyes.

Voelter, Katrin; Tappeiner, Christoph; Klein, Karina; Borel, Nicole; Bruetsch, Deborah; Sanz, Fernando Laguna; Pot, Simon Anton (2019). Fibrinolytic Capacity of Desmoteplase Compared to Tissue Plasminogen Activator in Rabbit Eyes. Journal of ocular pharmacology and therapeutics, 35(1), pp. 66-75. Mary Ann Liebert 10.1089/jop.2018.0070

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PURPOSE

Desmoteplase (DSPA) was evaluated and compared with tissue plasminogen activator (t-PA) for its intraocular fibrinolytic effect and short-term toxicity in an in vivo study using rabbit eyes.

METHODS

Fibrin clots were induced in the anterior chamber of 44 rabbit eyes, and drug efficacy was measured by clot size reduction over 24 h. Topical DSPA eye drops (1.4 and 2 mg/mL) were compared with vehicle solution in a multiple-drop regimen in 8 animals per group. Intracameral injections of 0.6 μg DSPA (n = 14) and 25 μg t-PA (n = 14) were evaluated for their fibrinolytic efficacy. Animals were euthanized 24 h after drug application.

RESULTS

No significant differences were seen between topically treated DSPA and vehicle-treated animals. Intracameral t-PA had a higher fibrinolytic efficacy than DSPA at early time points, but no significant difference was seen between both groups at 24-h postapplication. Animals with t-PA treatment demonstrated significantly more side effects compared with DSPA-treated animals. DSPA showed no-to-mild side effects after topical and intracameral treatment. Histologically, no toxic effects were observed in any globe.

CONCLUSIONS

DSPA is a promising drug with fewer side effects and similar fibrinolytic efficacy compared with t-PA 24 h after intracameral application in rabbit eyes at the tested concentration. Drug efficacy might be improved by increasing intracameral DSPA doses.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology

UniBE Contributor:

Tappeiner, Christoph

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1080-7683

Publisher:

Mary Ann Liebert

Language:

English

Submitter:

Christoph Tappeiner

Date Deposited:

01 Feb 2019 11:11

Last Modified:

17 Nov 2019 02:26

Publisher DOI:

10.1089/jop.2018.0070

PubMed ID:

30376399

Uncontrolled Keywords:

desmoteplase fibrin t-PA uveitis

BORIS DOI:

10.7892/boris.123074

URI:

https://boris.unibe.ch/id/eprint/123074

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