Acute effects of multi-walled carbon nanotubes on primary bronchial epithelial cells from COPD patients.

Beyeler, Seraina Martina; Chortarea, Savvina; Rothen-Rutishauser, Barbara; Petri-Fink, Alke; Wick, Peter; Tschanz, Stefan A.; von Garnier, Christophe; Blank, Fabian (2018). Acute effects of multi-walled carbon nanotubes on primary bronchial epithelial cells from COPD patients. Nanotoxicology, 12(7), pp. 699-711. Taylor & Francis 10.1080/17435390.2018.1472310

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The risks of occupational exposure during handling of multi-walled carbon nanotubes (MWCNTs) have received limited attention to date, in particular for potentially susceptible individuals with highly prevalent chronic obstructive pulmonary disease (COPD). In this in vitro study, we simulated acute inhalation of MWCNTs employing an air-liquid interface cell exposure (ALICE) system: primary human bronchial epithelial cells from COPD patients and healthy donors (controls), cultured at the air-liquid interface (ALI) were exposed to MWCNTs. To study acute health effects on the respiratory epithelium, two different concentrations (0.16; 0.34 µg/cm) of MWCNTs were aerosolized onto cell cultures followed by analysis after 24 h. Following MWCNT exposure, epithelial integrity and differentiation remained intact. Electron microscopy analyses identified MWCNTs both extra- and intracellular within vesicles of mucus producing cells. In both COPD and healthy control cultures, MWCNTs neither caused increased release of lactate dehydrogenase (LDH), nor alterations in inflammatory responses, as measured by RNA expression and protein secretion of the cytokines IL-6, IL-8, CXCL10, IL-1β and TGF-β and oxidative stress markers HMOX-1 and SOD-2. No short-term alteration of epithelial cell function, as determined by ciliary beating frequency (CBF), occurred in any of the conditions tested. In conclusion, the present study provided a reliable and realistic in vitro acute-exposure model of the respiratory tract, responsive to positive controls such as Dörentruper Quartz (DQ12) and asbestos. Acute exposure to MWCNTs did not affect epithelial integrity, nor induce increased cell death, apoptosis or inflammatory changes.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)
09 Interdisciplinary Units > Microscopy Imaging Center MIC

UniBE Contributor:

Beyeler, Seraina Martina; Tschanz, Stefan A.; von Garnier, Christophe and Blank, Fabian

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1743-5390

Publisher:

Taylor & Francis

Language:

English

Submitter:

Rahel Holderegger

Date Deposited:

14 Jan 2019 11:51

Last Modified:

14 Feb 2019 13:55

Publisher DOI:

10.1080/17435390.2018.1472310

PubMed ID:

29804489

Uncontrolled Keywords:

Exposure air–liquid interface cell exposure system fiber toxicology nanoparticles nanotubes

URI:

https://boris.unibe.ch/id/eprint/123141

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