Method for analysis of electrospray for gene transfer and the impact on cell viability of A549 alveolar epithelial like cells.

Hradetzky, David; Boehringer, Stephan; Ruzgys, Paulius; Satkauskas, Saulius; Geiser, Thomas; Gazdhar, Amiq (2018). Method for analysis of electrospray for gene transfer and the impact on cell viability of A549 alveolar epithelial like cells. IEEE Engineering in Medicine and Biology Society conference proceedings, 2018, pp. 5725-5728. IEEE Service Center 10.1109/EMBC.2018.8513643

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Electrospray is a process based on creation and acceleration of small sized droplets based on electrostatic repulsion. Spraying plasmid containing liquids this process may be used to transfer genes into cells. Within this paper we report on a method for accessing and evaluating the spray modalities using high speed imaging system with a post processing of image data to obtain estimated volume and velocity of emerging droplets first. Second we investigate on the impact of different media on the spray modalities. Third we evaluate the impact of the spray on cell viability and on transfection efficiency of an eGFP plasmid as reporter gene obtained in an in vitro setup on alveolar epithelial like cells (A549).

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology

UniBE Contributor:

Ruzgys, Paulius; Geiser, Thomas and Gazdhar, Amiq

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1557-170X

Publisher:

IEEE Service Center

Language:

English

Submitter:

Rahel Holderegger

Date Deposited:

07 Feb 2019 15:21

Last Modified:

07 Feb 2019 15:26

Publisher DOI:

10.1109/EMBC.2018.8513643

PubMed ID:

30441636

BORIS DOI:

10.7892/boris.123213

URI:

https://boris.unibe.ch/id/eprint/123213

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