Distribution of polymer-coated gold nanoparticles in a 3D lung model and indication of apoptosis after repeated exposure.

Chortarea, Savvina; Fytianos, Kleanthis; Rodriguez-Lorenzo, Laura; Petri-Fink, Alke; Rothen-Rutishauser, Barbara (2018). Distribution of polymer-coated gold nanoparticles in a 3D lung model and indication of apoptosis after repeated exposure. Nanomedicine, 13(10), pp. 1169-1185. Future Medicine 10.2217/nnm-2017-0358

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AIM The distribution and impact of aerosol-delivered gold nanoparticles (AuNPs) functionalized with a mixture of aminated-polyvinyl alcohol and amino-PEG ([polyvinyl alcohol/PEG]-NH) upon repeated administration onto a 3D lung model were explored. MATERIALS & METHODS AuNPs were aerosolized and uptake and epithelial translocation was assessed by inductively coupled plasma optical-emission spectroscopy, flow cytometry and electron microscopy. In addition, cytotoxicity, apoptosis and proinflammation were evaluated. RESULTS Repeated AuNP aerosolization resulted in NP accumulation in macrophages and epithelial cells. Dendritic cells demonstrated substantial NP internalization after single administration which was reduced in later time points. No cytotoxicity or proinflammation was observed but after 96 h significant apoptosis was induced by the polymer coating. CONCLUSION These results indicate the importance of repeated exposures in addressing potential effects of NPs.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology

UniBE Contributor:

Fytianos, Kleanthis

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1748-6963

Publisher:

Future Medicine

Language:

English

Submitter:

Rahel Holderegger

Date Deposited:

08 Feb 2019 09:42

Last Modified:

08 Feb 2019 09:50

Publisher DOI:

10.2217/nnm-2017-0358

PubMed ID:

29874145

Uncontrolled Keywords:

air–liquid interface biodistribution biomedical gold nanoparticles hazard assessment in vitro 3D lung system short-term repeated aerosol exposure

BORIS DOI:

10.7892/boris.123223

URI:

https://boris.unibe.ch/id/eprint/123223

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