Complement Deposition and IgG Binding on Stored Red Blood Cells Are Independent of Storage Time.

Thielen, Astrid J F; Meulenbroek, Elisabeth M; Baas, Inge; Bruggen, Robin; Zeerleder, Sacha S.; Wouters, Diana (2018). Complement Deposition and IgG Binding on Stored Red Blood Cells Are Independent of Storage Time. Transfusion medicine and hemotherapy, 45(6), pp. 378-384. Karger 10.1159/000486759

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Background In the Netherlands, red blood cells (RBCs) are allowed to be stored up to 35 days at 2-6 °C in saline-adenine-glucose-mannitol (SAGM). During storage, RBCs undergo several changes that are collectively known as storage lesion. We investigated to what extent complement deposition and antibody binding occurred during RBC storage and investigated phagocytic uptake in vitro. Methods RBCs were stored for different lengths of time at 2-6 °C in SAGM. Complement deposition and antibody binding were assessed upon storage and after incubation with serum. M1- and M2-type macrophages were generated from blood monocytes to investigate RBC phagocytosis. Results No complement deposition was directly observed on stored RBCs, while incubation of RBCs with serum resulted in variable donor-dependent C3 deposition and IgG binding, both independent of storage time. Only 1-4% phagocytosis of stored RBCs by macrophages was observed. Conclusion RBCs are susceptible to complement deposition and antibody binding independent of storage time. Limited phagocytic uptake by macrophages was observed in vitro.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Haematology and Central Haematological Laboratory

UniBE Contributor:

Zeerleder, Sacha Sergio

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1660-3796

Publisher:

Karger

Language:

English

Submitter:

Pierrette Durand Lüthi

Date Deposited:

08 Jan 2019 12:16

Last Modified:

22 Oct 2019 19:30

Publisher DOI:

10.1159/000486759

PubMed ID:

30574054

Uncontrolled Keywords:

Antibody binding Complement activation Phagocytosis Red blood cells Storage

BORIS DOI:

10.7892/boris.123251

URI:

https://boris.unibe.ch/id/eprint/123251

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